中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2022, Vol. 17 ›› Issue (6): 1286-1292.doi: 10.4103/1673-5374.327354

• 原著:神经损伤修复保护与再生 • 上一篇    下一篇

成年小鼠齿状回中成熟/未成熟神经元、胶质细胞和内皮细胞的密度

  

  • 出版日期:2022-06-15 发布日期:2021-12-17

Estimation of the density of neural, glial, and endothelial lineage cells in the adult mouse dentate gyrus

Joshua D. Rieskamp1, 2, Patricia Sarchet2, Bryon M. Smith2, Elizabeth D. Kirby2, 3, 4, *   

  1. 1Neuroscience Graduate Program, 2Department of Psychology, 3Department of Neuroscience, 4Chronic Brain Injury Program, The Ohio State University, Columbus, OH, USA
  • Online:2022-06-15 Published:2021-12-17
  • Contact: Elizabeth D. Kirby, PhD, kirby.224@osu.edu.
  • Supported by:
    The study was partially supported by a R00 Pathway to Independence Award from NIH/NINDS (R00NS089938; to EDK).

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摘要：

哺乳动物海马的齿状回（DG）亚区是成体神经干细胞龛和终身神经发生的场所。通过确定成年出生后与产前/围产期新生神经元数量可了解成年出生后新生神经元突触整合在海马回路功能中的作用。实验应用免疫组化方法估计神经干/祖细胞和其他主要的胶质和内皮细胞的密度，这些细胞在成年小鼠的DG中是潜在的分泌性细胞。量化分析显示，GFAP+SOX2+星状星形细胞是最多的，其次是CD31+内皮细胞、GFAP-SOX2+中间祖细胞、Olig2+少突胶质细胞、Iba1+小胶质细胞和GFAP+SOX2+放射状胶质样神经干细胞。实验未观察到任何细胞群的密度有明显的性别差异。值得注意的是，神经干/祖细胞的密度与几种已知通过其分泌组具有强大功能作用的细胞相似。这些发现将有助于理解这些细胞类型在调节海马功能中的贡献。

https://orcid.org/0000-0001-9313-3790 (Elizabeth D. Kirby)

Abstract: The dentate gyrus subregion of the mammalian hippocampus is an adult neural stem cell niche and site of lifelong neurogenesis. Hypotheses regarding the role of adult-born neuron synaptic integration in hippocampal circuit function are framed by robust estimations of adult-born versus pre/perinatally-born neuron number. In contrast, the non-neurogenic functions of adult neural stem cells and their immediate progeny, such as secretion of bioactive growth factors and expression of extracellular matrix-modifying proteins, lack similar framing due to few estimates of their number versus other prominent secretory cells. Here, we apply immunohistochemical methods to estimate cell density of neural stem/progenitor cells versus other major classes of glial and endothelial cell types that are potentially secretory in the dentate gyrus of adult mice. Of the cell types quantified, we found that GFAP+SOX2+ stellate astrocytes were the most numerous, followed by CD31+ endothelia, GFAP–SOX2+ intermediate progenitors, Olig2+ oligodendrocytes, Iba1+ microglia, and GFAP+SOX2+ radial glia-like neural stem cells. We did not observe any significant sex differences in density of any cell population. Notably, neural stem/progenitor cells were present at a similar density as several cell types known to have potent functional roles via their secretome. These findings may be useful for refining hypotheses regarding the contributions of these cell types to regulating hippocampal function and their potential therapeutic uses. All experimental protocols were approved by the Ohio State University Institutional Animal Care and Use Committee (protocol# 2016A00000068) on July 14, 2016.

Key words: adult neurogenesis, dentate gyrus, endothelia, glia, hippocampus, neural stem cell, secretome, stereology

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