中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2026, Vol. 21 ›› Issue (3): 1183-1190.doi: 10.4103/NRR.NRR-D-24-00128

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

低强度经颅聚焦超声刺激治疗缺血性脑卒中：基于电阻抗断层成像技术的体内评估

  

  • 出版日期:2026-03-15 发布日期:2025-07-05

Therapeutic effects of low-intensity transcranial focused ultrasound stimulation on ischemic stroke in rats: An in vivo evaluation using electrical impedance tomography

Jiecheng Guo1, #, Sixuan He1, #, Li Yan1 , Lei Wang1 , Xuetao Shi2 , Huijing Hu1, *, Le Li1, *   

  1. 1 Xi’an Key Laboratory of Stem Cell and Regenerative Medicine, Institute of Medical Research, Northwestern Polytechnical University, Xi’an, Shaanxi Province, China;  2 Department of Biomedical Engineering, Air Force Medical University, Xi’an, Shaanxi Province, China
  • Online:2026-03-15 Published:2025-07-05
  • Contact: Huijing Hu, PhD, huhuijing@nwpu.edu.cn; Le Li, PhD, lile5@nwpu.edu.cn.
  • Supported by:
    This study was supported by the Fundamental Research Funds for the Central Universities, Nos. G2021KY05107, G2021KY05101; the National Natural Science Foundation of China, Nos. 32071316, 32211530049; the Natural Science Foundation of Shaanxi Province, No. 2022-JM482; and the Education and Teaching Reform Funds for the Central Universities, No. 23GZ230102 (all to LL and HH).

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摘要：

既往研究表明，经颅聚焦超声刺激对脑缺血有保护作用，但仍缺乏定量评估和机制探索。而电阻抗断层成像技术可实时监测脑缺血颅内血流灌注的变化，因而可将其应用于评估经颅聚焦超声刺激干预的效果。此次实验首先以大脑中动脉阻塞法诱导建立脑缺血大鼠模型，随后对损伤侧进行连续3d，每天20min的经颅聚焦超声刺激治疗。电阻抗断层成像技术观察可见，脑缺血后大脑阻抗增加，而经颅聚焦超声刺激后其阻抗降低。同时经颅聚焦超声刺激还可促进缺血性脑卒中大鼠运动功能的恢复，缩小脑梗死体积，并诱导脑组织中脑源性神经营养因子的表达。此外，经颅聚焦超声刺激后大鼠脑阻抗与mNSS行为学评分和梗死体积呈正相关。由此提示，经颅聚焦超声刺激可通过缩小脑损伤体积并促进脑源性神经营养因子的表达，促进缺血性脑卒中的恢复，且电阻抗断层成像技术可作为定量评估缺血性脑卒中康复效果的有力工具。

https://orcid.org/0000-0002-9340-9061 (Le Li); https://orcid.org/0000-0001-6639-6763 (Huijing Hu)

关键词: 经颅聚焦超声刺激, 电阻抗断层成像技术, 脑卒中, 康复, 动物模型, 阻抗, 脑刺激, 评估, 实时监控, 非侵入性治疗

Abstract: Although previous studies have demonstrated that transcranial focused ultrasound stimulation protects the ischemic brain, clear criteria for the stimulation time window and intensity are lacking. Electrical impedance tomography enables real-time monitoring of changes in cerebral blood perfusion within the ischemic brain, but investigating the feasibility of using this method to assess post-stroke rehabilitation in vivo remains critical. In this study, ischemic stroke was induced in rats through middle cerebral artery occlusion surgery. Transcranial focused ultrasound stimulation was used to treat the rat model of ischemia, and electrical impedance tomography was used to measure impedance during both the acute stage of ischemia and the rehabilitation stage following the stimulation. Electrical impedance tomography results indicated that cerebral impedance increased after the onset of ischemia and decreased following transcranial focused ultrasound stimulation. Furthermore, the stimulation promoted motor function recovery, reduced cerebral infarction volume in the rat model of ischemic stroke, and induced the expression of brain-derived neurotrophic factor in the ischemic brain. Our results also revealed a significant correlation between the impedance of the ischemic brain post-intervention and improvements in behavioral scores and infarct volume. This study shows that daily administration of transcranial focused ultrasound stimulation for 20 minutes to the ischemic hemisphere 24 hours after cerebral ischemia enhanced motor recovery in a rat model of ischemia. Additionally, our findings indicate that electrical impedance tomography can serve as a valuable tool for quantitatively evaluating rehabilitation after ischemic stroke in vivo. These findings suggest the feasibility of using impedance data collected via electrical impedance tomography to clinically assess the effects of rehabilitatory interventions for patients with ischemic stroke.

Key words: animal model, brain stimulation, electrical impedance tomography, evaluation, impedance, noninvasive treatment, real-time monitoring, rehabilitation, stroke, transcranial focused ultrasound stimulation