中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (24): 2132-2141.doi: 10.4103/1673-5374.147944

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

一种新的人工神经修复长距离坐骨神经缺损：聚乳酸-羟基乙酸共聚物多肽自组装纳米纤维导管

  

  • 收稿日期:2014-10-06 出版日期:2014-12-25 发布日期:2014-12-25
  • 基金资助:

    973课题(2014CB542202 and 2014CB542205)；国家自然科学基金(30973095 & 81371354)；广东省科技术项目(12C32121609)；广东省自然科学基金(S2013010014697)

A novel artificial nerve graft for repairing long-distance sciatic nerve defects: a self-assembling peptide nanofiber scaffold-containing poly(lactic-co-glycolic acid) conduit

Xianghai Wang 1, Mengjie Pan 1, Jinkun Wen 1, Yinjuan Tang 2, Audra D. Hamilton 3, Yuanyuan Li 1, Changhui Qian 1, Zhongying Liu 1, Wutian Wu 4, 5, Jiasong Guo 1, 6,  7   

  1. 1 Department of Histology and Embryology, Southern Medical University, Guangzhou, Guangdong Province, China
    2 Department of Histology and Embryology, Xiangnan University, Chenzhou, Hunan Province, China
    3 Department of Neurology, Vanderbilt University, Nashville, TN, USA
    4 Department of Anatomy, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China
    5 GHM Institute of CNS Regeneration, Jinan University, Guangzhou, Guangdong Province, China
    6 Key Laboratory of Tissue Construction and Detection of Guangdong Province, Guangzhou, Guangdong Province, China
    7 Institute of Bone Biology, Academy of Orthopedics, Guangzhou, Guangdong Province, China
  • Received:2014-10-06 Online:2014-12-25 Published:2014-12-25
  • Contact: Jiasong Guo, Department of Histology and Embryology, Southern Medical University, Guangzhou 510515, China, jiasongguo@aliyun.com.
  • Supported by:

    This work was supported by a grant from the National Key Basic Research Program of China, No. 2014CB542202 and 2014CB542205; the National Natural Science Foundation of China, No. 30973095 & 81371354; a grant from Science and Technology Project of Guangzhou, in China, No. 12C32121609; the Natural Science Foundation of Guangdong Province of China, No. S2013010014697 to Guo JS and Hong Kong SCI Fund to Wu WT.

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摘要：

我们构建了一种新型的聚乳酸-羟基乙酸共聚物多肽自组装纳米纤维人工神经导管，用其桥接修复大鼠10mm坐骨神经缺损。神经逆行示踪、行为学评估及一系列组织形态学测定结果显示，与移植空聚乳酸-羟基乙酸共聚物导管比较，移植的聚乳酸-羟基乙酸共聚物多肽自组装纳米纤维人工神经导管中有更多的轴突生长和穿越，且再生轴突直径、导管中髓鞘厚度、神经肌肉接头尺寸、靶肌肉肌纤维直径明显增加。说明我们设计实验成功的聚乳酸-羟基乙酸共聚物多肽自组装纳米纤维人工神经导管，可促进缺损周围神经轴突再生和再髓鞘化，可用于修复损伤的周围神经。

关键词: 神经再生, 周围神经损伤, 人工神经移植, 聚乳酸-羟基乙酸共聚物, 自组装多肽, 纳米纤维支架, 再髓鞘化, 轴突, 髓鞘, 神经肌肉接头

Abstract:

In this study, we developed a novel artificial nerve graft termed self-assembling peptide nanofiber scaffold (SAPNS)-containing poly(lactic-co-glycolic acid) (PLGA) conduit (SPC) and used it to bridge a 10-mm-long sciatic nerve defect in the rat. Retrograde tracing, behavioral testing and histomorphometric analyses showed that compared with the empty PLGA conduit implantation group, the SPC implantation group had a larger number of growing and extending axons, a markedly increased diameter of regenerated axons and a greater thickness of the myelin sheath in the conduit. Furthermore, there was an increase in the size of the neuromuscular junction and myofiber diameter in the target muscle. These findings suggest that the novel artificial SPC nerve graft can promote axonal regeneration and remyelination in the transected peripheral nerve and can be used for repairing peripheral nerve injury.

Key words: nerve regeneration, peripheral nerve defect, artificial nerve graft, poly(lactic-co-glycolic acid), self-assembling peptide, nanofiber scaffold, remyelination, axon, myelin, neuromuscular junction, NSFC grants, neural regeneration