中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (24): 2142-2150.doi: 10.4103/1673-5374.147948

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

牛膝多肽k有望成为促进神经元生长和挤压伤周围神经再生的潜力药物

  

  • 收稿日期:2014-10-10 出版日期:2014-12-25 发布日期:2014-12-25
  • 基金资助:

    973课题(2014CB542202);江苏省高校优势学科资助项目

The Achyranthes bidentata polypeptide k fraction enhances neuronal growth in vitro and promotes peripheral nerve regeneration after crush injury in vivo

Qiong Cheng, Chunyi Jiang, Caiping Wang, Shu Yu, Qi Zhang, Xiaosong Gu, Fei Ding   

  1. Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregenration, Nantong University, Nantong, Jiangsu Province, China
  • Received:2014-10-10 Online:2014-12-25 Published:2014-12-25
  • Contact: Xiaosong Gu, Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregenration, Nantong University, 19 Qixiu Road, Nantong 226001, Jiangsu Province, China, nervegu@ntu.edu.cn. Fei Ding, Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregenration, Nantong University, 19 Qixiu Road, Nantong 226001, Jiangsu Province, China, dingfei@ntu.edu.cn.
  • Supported by:

    This study was supported by a grant from National Key Basic Research Program of China (973 Program), No. 2014CB542202; a grant from Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) in China.

摘要:

我们前期研究已证实,提取自苋科植物牛膝的牛膝多肽对神经系统有神经营养和保护作用。为了确认牛膝多肽主要活性成分,以便使牛膝多肽达到最佳应用效果。应用反相高效液相色谱法分离牛膝多肽,获得12个片段,证实片段之一牛膝多肽k神经活性最强。免疫组和免疫印迹分析结果显示,牛膝多肽k可能通过激活细胞外信号调节激酶1/2,促进培养的背根神经节组织块及其神经元突起生长。行为学、电生理学和组织形态学分析显示,牛膝多肽k可促进坐骨神经挤压伤小鼠模型神经再生。这些结果验证了牛膝多肽k对周围神经损伤的良好修复效果。
 

关键词: 神经再生, 牛膝多肽, 神经活性成分, 背根神经节, 神经突起生长, 挤压伤, 坐骨神经, 周围神经损伤

Abstract:

We have previously shown that Achyranthes bidentata polypeptides (ABPP), isolated from Achyranthes bidentata Blume (a medicinal herb), exhibit neurotrophic and neuroprotective effects on the nervous system. To identify the major active component of ABPP, and thus optimize the use of ABPP, we used reverse-phase high performance liquid chromatography to separate ABPP. We obtained 12 fractions, among which the fraction of ABPPk demonstrated the strongest neuroactivity. Immunocytochemistry and western blot analysis showed that ABPPk promoted neurite growth in cultured dorsal root ganglion explant and dorsal root ganglion neurons, which might be associated with activation of Erk1/2. A combination of behavioral tests, electrophysiological assessment, and histomorphometric analysis indicated that ABPPk enhanced nerve regeneration and function restoration in a mouse model of crushed sciatic nerve. All the results suggest that ABPPk, as the key component of ABPP, can be used for peripheral nerve repair to yield better outcomes than ABPP.

Key words: nerve regeneration, Achyranthes bidentata polypeptides, neuroactive component, dorsal root ganglion, neurite outgrowth, crush injury, sciatic nerve, peripheral nerve regeneration, neural regeneration