中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (10): 1635-1642.doi: 10.4103/1673-5374.167763

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

电纺丝与编织法制备的丝素蛋白/聚乳酸聚乙醇酸神经导管可用于修复周围神经缺损?

  

  • 收稿日期:2015-06-10 出版日期:2015-10-28 发布日期:2015-10-28
  • 基金资助:

    国家自然科学基金(81371687, 81171457)

Electrospun and woven silk fibroin/poly(lactic-co- glycolic acid) nerve guidance conduits for repairing peripheral nerve injury

Ya-ling Wang1, 2, Xiao-mei Gu3, Yan Kong4, Qi-lin Feng5, Yu-min Yang1, 4, *   

  1. 1 Key Laboratory of Eco-Textiles, Ministry of Education, Jiangnan University, Wuxi, Jiangsu Province, China
    2 School of Chemistry and Chemical Engineering, Nantong University, Nantong, Jiangsu Province, China
    3 Jiangsu College of Engineering and Technology, Nantong, Jiangsu Province, China
    4 Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, Jiangsu Province, China
    5 School of Medicine, Nantong University, Nantong, Jiangsu Province, China
  • Received:2015-06-10 Online:2015-10-28 Published:2015-10-28
  • Contact: Yu-min Yang, M.D., yangym@ntu.edu.cn.
  • Supported by:

    This study was supported by the National Natural Science Foundation of China, No. 81371687, 81171457.

摘要:

应用电纺丝与编织法制备的由丝素蛋白和聚乳酸聚乙醇酸构建的新型神经导管,是否具备临床用于损伤周围神经的修复的潜质?我们以体外实验评估物理特性包括厚度、机械特性、红外光谱、多孔性和吸水性。在构建的丝素蛋白-聚乳酸聚乙醇酸神经导管支架材料上种植许旺细胞后,进行细胞形态观察,MTT和免疫组化检测,以评估导管的细胞生物相容性。将丝素蛋白/聚乳酸聚乙醇酸神经移植物种植于兔后背皮下,以评估其体内生物相容性。结果见丝素蛋白/聚乳酸聚乙醇酸神经导管具有多孔性、强力亲水性和良好的机械性能;培养于丝素蛋白/聚乳酸聚乙醇酸神经导管支架材料提取液中的许旺细胞与培养与完全培养基培养的许旺细胞形态和活性相近;体内组织学评估显示丝素蛋白/聚乳酸聚乙醇酸神经移植物诱发轻微炎症反应,生物相容性较好。上述结果可以验证电纺丝与编织法制备的新型丝素蛋白/聚乳酸聚乙醇酸神经导管表现出良好的生物相容性,具有修复周围神经损伤的临床转化应用前景。

关键词: 神经再生, 周围神经损伤, PLGA, 生物相容性, 神经导管, 电纺丝, 编织

Abstract:

We have designed a novel nerve guidance conduit (NGC) made from silk fibroin and poly(lactic-co-glycolic acid) through electrospinning and weaving (ESP-NGCs). Several physical and biological properties of the ESP-NGCs were assessed in order to evaluate their biocompatibility. The physical properties, including thickness, tensile stiffness, infrared spectroscopy, porosity, and water absorption were determined in vitro. To assess the biological properties, Schwann cells were cultured in ESP-NGC extracts and were assessed by morphological observation, the MTT assay, and immunohistochemistry. In addition, ESP-NGCs were subcutaneously implanted in the backs of rabbits to evaluate their biocompatibility in vivo. The results showed that ESP-NGCs have high porosity, strong hydrophilicity, and strong tensile stiffness. Schwann cells cultured in the ESP-NGC extract fluids showed no significant differences compared to control cells in their morphology or viability. Histological evaluation of the ESP-NGCs implanted in vivo indicated a mild inflammatory reaction and high biocompatibility. Together, these data suggest that these novel ESP-NGCs are biocompatible, and may thus provide a reliable scaffold for peripheral nerve repair in clinical application.

Key words: nerve regeneration, peripheral nerve injury, poly(lactic-co-glycolic acid), electrospinning, silk fibroin, biocompatibility, nerve guidance conduit, weaving