中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (10): 1628-1634.doi: 10.4103/1673-5374.167762

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

电针刺激保护损伤脊髓的早期和晚期效应

  

  • 收稿日期:2015-08-21 出版日期:2015-10-28 发布日期:2015-10-28
  • 基金资助:

    吉林省科技发展计划资助项目(2011084)

Neuroprotective effects of electroacupuncture on early- and late-stage spinal cord injury

Min-fei Wu1, Shu-quan Zhang2, Jia-bei Liu3, Ye Li3, Qing-san Zhu3, Rui Gu3, *   

  1. 1 Department of Orthopedics, Bethune Second Hospital of Jilin University, Changchun, Jilin Province, China
    2 Department of Orthopedics, Tianjin Nankai Hospital, Tianjin, China
    3 Department of Orthopedics, China-Japan Union Hospital, Jilin University, Changchun, Jilin Province, China
  • Received:2015-08-21 Online:2015-10-28 Published:2015-10-28
  • Contact: Rui Gu, M.D., fandiliucl@126.com.
  • Supported by:

    This study was supported by a grant from the Science and Technology Development Program
    of Jilin Province of China, No. 2011084.

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摘要：

已知Nogo-A能通过激活RhoA等引起继发性脑损伤，我们假设电针刺激可抑制该表达产生对脊髓损伤后神经功能恢复的影响，为此实验按照改良的Allen打击法建立急性脊髓损伤模型大鼠，6h后起采用督脉(大椎和命门)加体穴(三阴交、环跳、足三里、昆仑穴)电针，疏密波，频率4Hz，1次/d，每次30 min，连续7d。损伤7d后，大鼠BBB评分及斜板试验评分显著增加，脊髓组织中凋亡细胞数量明显减少，RhoA和Nogo-A mRNA及蛋白的表达降低；4周后，大鼠脊髓损伤区域的病理损伤减轻，胶质纤维酸性蛋白及神经丝蛋白200阳性纤维数量增加，体感诱发电位和运动诱发电位潜伏期缩短，波幅增高。表明电针刺激治疗减少了大鼠脊髓损伤区神经细胞的凋亡和降低RhoA、Nogo-A基因和蛋白的表达，促进了脊髓损伤区域的神经修复和神经功能恢复。

关键词: 脊髓损伤, 电针刺激, 神经再生, 运动功能, 大鼠, RhoA, Nogo-A, 胶质纤维酸性蛋白, 神经丝蛋白200

Abstract:

Previous studies have shown that the neurite growth inhibitor Nogo-A can cause secondary neural damage by activating RhoA. In the present study, we hypothesized that electroacupuncture promotes neurological functional recovery after spinal cord injury by inhibiting RhoA expression. We established a rat model of acute spinal cord injury using a modification of Allen’s method. The rats were given electroacupuncture treatment at Dazhui (Du14), Mingmen (Du4), Sanyinjiao (SP6), Huantiao (GB30), Zusanli (ST36) and Kunlun (BL60) acupoints with a sparse-dense wave at a frequency of 4 Hz for 30 minutes, once a day, for a total of 7 days. Seven days after injury, the Basso, Beattie and Bresnahan (BBB) locomotor scale and inclined plane test scores were significantly increased, the number of apoptotic cells in the spinal cord tissue was significantly reduced, and RhoA and Nogo-A mRNA and protein expression levels were decreased in rats given electroacupuncture compared with rats not given electroacupuncture. Four weeks after injury, pathological tissue damage in the spinal cord at the site of injury was alleviated, the numbers of glial fibrillary acidic protein- and neurofilament 200-positive fibers were increased, the latencies of somatosensory-evoked and motor-evoked potentials were shortened, and their amplitudes were increased in rats given electroacupuncture. These findings suggest that electroacupuncture
treatment reduces neuronal apoptosis and decreases RhoA and Nogo-A mRNA and protein expression at the site of spinal cord injury, thereby promoting tissue repair and neurological functional recovery.

Key words: nerve regeneration, spinal cord injury, electroacupuncture, locomotion, RhoA, Nogo-A, glial fibrillary acidic protein, neurofilament 200, neural regeneration