中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (2): 244-251.doi: 10.4103/1673-5374.152378

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

抗脊髓散发胶质瘤发生：Notch通路可成为治疗新靶点？

  

  • 收稿日期:2014-10-04 出版日期:2015-02-17 发布日期:2015-02-17
  • 基金资助:

    国家教育部优秀青年教师基金资助(200800011035)，国家自然科学基金(81200969/H0912)

     

Prevention against diffuse spinal cord astrocytoma: can the Notch pathway be a novel treatment target?

Jian-jun Sun 1, Zhen-yu Wang 1, Ling-song Li 2, Hai-yan Yu 3, Yong-sheng Xu 3, 4, Hai-bo Wu 5, Yi Luo 1, Bin Liu 1, Mei Zheng 6, Jin-long Mao 7, Xiao-hui Lou 8   

  1. 1 Department of Neurosurgery, Peking University Third Hospital, Peking University, Beijing, China
    2 China Stem Cell Research Center, Peking University Health Science Center, Peking University, Beijing, China
    3 Clinical Stem Cell Center, Peking University Third Hospital, Peking University, Beijing, China
    4 Clinical Laboratory of Tissue & Cell Research Center, Department of Biotech Treatment, Logistics College of Chinese People’s Armed Police Force, Tianjin, China
    5 Department of Neuroradiology, Peking University Third Hospital, Peking University, Beijing, China
    6 Department of Neurology, Peking University Third Hospital, Peking University, Beijing, China
    7 Neurosurgical Department, Peking Union Medical College Hospital, Beijing, China
    8 Department of Neurosurgery, Rui’an People’s Hospital, Wenzhou Medical University, Wenzhou, Zhejiang Province, China
  • Received:2014-10-04 Online:2015-02-17 Published:2015-02-17
  • Contact: Jian-jun Sun, M.D. or Xiao-hui Lou, M.D., sunjj2008@gmail.com or louxiaohui@163.com.
  • Supported by:

    This work was supported by grants from Science Foundation of Ministry of Education of China for the Excellent Youth Scholars, No. 200800011035, and the National Natural Science Foundation of China, No. 81200969/H0912.

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摘要：

实验为确认Notch信号通路是否参与脊髓星形胶质细胞瘤的形成。为些，实验中分别给予BALB/c裸小鼠大脑注射来源于人复发脊髓星形胶质瘤CD133阳性和阴性细胞悬液。注射后7-11周，每周进行MRI检测，发现移植CD133阳性胶质瘤细胞小鼠大脑表面形成移植瘤，移植瘤Notch免疫阳性表达；移植CD133阴性胶质瘤细胞小鼠大脑表面无移植瘤形成，几乎未见Notch免疫阳性表达。HE和免疫组化染色检测到移植CD133阳性胶质瘤细胞的小鼠大脑凸面形成移植瘤。一些散发的星形胶质瘤细胞形成伪足样结构浸润大脑白质。说明Notch通路在星形胶质细胞瘤的形成中有重要作用，可成为今后治疗脊髓星形胶质细胞瘤的新靶点？

关键词: 星形胶质细胞瘤, 小鼠, 免疫缺陷鼠, Notch, nestin, 神经胶质纤维酸性蛋白, CD133, 脊髓, 大脑, MRI

Abstract:

This study was designed to investigate whether the Notch pathway is involved in the development of diffuse spinal cord astrocytomas. BALB/c nude mice received injections of CD133+ and CD133− cell suspensions prepared using human recurrent diffuse spinal cord astrocytoma tissue through administration into the right parietal lobe. After 7–11 weeks, magnetic resonance imaging was performed weekly. Xenografts were observed on the surfaces of the brains of mice receiving the CD133+ cell suspension, and Notch-immunopositive expression was observed in the xenografts. By contrast, no xenografts appeared in the identical position on the surfaces of the brains of mice receiving the CD133− cell suspension, and Notch-immunopositive expression was hardly detected either. Hematoxylin-eosin staining and immunohistochemical staining revealed xenografts on the convex surfaces of the brains of mice that underwent CD133+ astrocytoma transplantation. Some sporadic astroglioma cells showed pseudopodium-like structures, which extended into the cerebral white matter. However, it should be emphasized that the subcortex xenograft with Notch-immunopositive expression was found in the fourth mouse received injection of CD133− astrocytoma cells. However, these findings suggest that the Notch pathway plays an important role in the formation of astrocytomas, and can be considered a novel treatment target for diffuse spinal cord astrocytoma.

Key words: nerve regeneration, astrocytoma, mice, immunodeficiency (BALB/c) mice, Notch, nestin, glial fibrillary acidic protein, CD133, spinal cord, brain, MRI, neural regeneration