中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (7): 1088-1094.doi: 10.4103/1673-5374.160100

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

提高液压冲击致脑创伤大鼠模型的稳定性：3种不同冲击力的比较

  

  • 收稿日期:2015-05-07 出版日期:2015-07-24 发布日期:2015-07-24

Stability of rat models of fluid percussion-induced traumatic brain injury: comparison of three different impact forces

Yun-peng Lin 1, 2, Rong-cai Jiang 1, Jian-ning Zhang 1   

  1. 1 Department of Neurosurgery, Tianjin Medical University General Hospital; Tianjin Neurological Institute; Key Laboratory of Post-trauma Neurorepair and Regeneration in Central Nervous System, Ministry of Education; Tianjin Key Laboratory of Injuries, Variations and Regeneration of Nervous System, Tianjin, China
    2 Tianjin Medical University General Hospital, Tianjin, China
  • Received:2015-05-07 Online:2015-07-24 Published:2015-07-24
  • Contact: Jian-ning Zhang,jianningzhang@hotmail.com.
  • Supported by:

    This work was supported by a grant from the International Science and Technology Cooperation Projects of China, No. 2011DFG33430.

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摘要：

液压冲击致脑创伤动物模型已在实验研究中广泛使用，但冲击力等因素不可避免的影响模型稳定性。控制冲击力的决定因素之一是控制压力和方向。为了提高实验的稳定性，实验首先建立了流体冲击装置的摆角-压力曲线，然后根据此曲线使用立体框架控制冲击方向，当摆角分别为6°，11°和13°，且冲击力分别为1.0 ± 0.11atm(101.32±11.16 kPa)，2.6 ± 0.16atm (263.44±16.21 kPa)和3.6 ± 0.16atm(364.77±16.21 kPa)时，可以稳定可重复的分别建立轻、中和重度颅脑损伤模型。行为学测试、HE染色及MRI检测结果显示，3种不同损伤程度的模型符合轻、中和重度颅脑损伤的临床特点。说明改进的液压冲击模型可以通过调整冲击力的大小和角度控制损伤程度，增加脑创伤动物模型的稳定性。

关键词: 神经再生, 脑创伤, 液压冲击, 冲击力, 压力曲线, 头部固定, 冲击峰, 动物模型

Abstract:

Fluid percussion-induced traumatic brain injury models have been widely used in experimental research for years. In an experiment, the stability of impaction is inevitably affected by factors such as the appearance of liquid spikes. Management of impact pressure is a crucial factor that determines the stability of these models, and direction of impact control is another basic element. To improve experimental stability, we calculated a pressure curve by generating repeated impacts using a fluid percussion device at different pendulum angles. A stereotactic frame was used to control the direction of impact. We produced stable and reproducible models, including mild, moderate, and severe traumatic brain injury, using the MODEL01-B device at pendulum angles of 6°, 11° and 13°, with corresponding impact force values of 1.0 ± 0.11 atm (101.32 ± 11.16 kPa), 2.6 ± 0.16 atm (263.44 ± 16.21 kPa), and 3.6 ± 0.16 atm (364.77 ± 16.21 kPa), respectively. Behavioral tests, hematoxylin-eosin staining, and magnetic resonance imaging revealed that models for different degrees of injury were consistent with the clinical properties of mild, moderate, and severe craniocerebral injuries. Using this method, we established fluid percussion models for different degrees of injury and stabilized pathological features based on precise power and direction control.

Key words: nerve regeneration, traumatic brain injury, fluid percussion, impact force, pressure curve, head fixed, impact peak, animal models, neural regeneration