中国神经再生研究(英文版) ›› 2022, Vol. 17 ›› Issue (7): 1588-1595.doi: 10.4103/1673-5374.330619

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

坐骨神经损伤后细胞衰老和增殖的剧烈时程变化

  

  • 出版日期:2022-07-15 发布日期:2022-01-18
  • 基金资助:
    国家自然科学基金项目(31970968);江苏省高等学校重点学科建设项目

Robust temporal changes of cellular senescence and proliferation after sciatic nerve injury

Yin-Ying Shen#, Rui-Rui Zhang#, Qian-Yan Liu, Shi-Ying Li*, Sheng Yi*   

  1. Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-innovation Center of Neuroregeneration, NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products, Nantong University, Nantong, Jiangsu Province, China
  • Online:2022-07-15 Published:2022-01-18
  • Contact: Shi-Ying Li, PhD, lisy0379@ntu.edu.cn; Sheng Yi, PhD, syi@ntu.edu.cn.
  • Supported by:
    This study was supported by the National Natural Science Foundation of China, No. 31970968 (to SYL); and Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

摘要:

细胞衰老和增殖对于伤口愈合和组织重塑至关重要。然而,周围神经损伤后细胞衰老和增殖的命运尚未被明确揭示。实验首先分析美国国家生物信息中心(NCBI)数据库收录的坐骨神经损伤大鼠坐骨神经残端(SRP113121)和L4-5背根神经节(SRP200823)中的转录组数据以破译细胞衰老和增殖相关的遗传变化。继而实验构建了坐骨神经挤压伤大鼠模型,通过β-半乳糖苷酶活性检测说明损伤的坐骨神经存在一定程度的细胞衰老,进而通过Ki67和EdU免疫染色说明损伤的坐骨神经存在明显激活的细胞增殖。背根神经节的变化相较于受损周围神经较弱。实验从遗传学和形态学方面阐明了损伤周围神经组织和背根神经节处由损伤诱导的细胞衰老和增殖的动态变化,扩展了对周围神经损伤后生物学变化过程的理解。研究于2019年2月26日经南通大学实验动物伦理委员会批准(批准号20190226-001)。

https://orcid.org/0000-0001-5150-7299 (Shi-Ying Li); https://orcid.org/0000-0003-1316-3370 (Sheng Yi)

关键词: 坐骨神经, 周围神经损伤, 坐骨神经挤压伤, 背根神经节, 细胞衰老, 细胞增殖, 周围神经再生, 生物信息学分析, β-半乳糖苷酶活性, p16

Abstract: Cellular senescence and proliferation are essential for wound healing and tissue remodeling. However, senescence-proliferation cell fate after peripheral nerve injury has not been clearly revealed. Here, post-injury gene expression patterns in rat sciatic nerve stumps (SRP113121) and L4–5 dorsal root ganglia (SRP200823) obtained from the National Center for Biotechnology Information were analyzed to decipher cellular senescence and proliferation-associated genetic changes. We first constructed a rat sciatic nerve crush model. Then, β-galactosidase activities were determined to indicate the existence of cellular senescence in the injured sciatic nerve. Ki67 and EdU immunostaining was performed to indicate cellular proliferation in the injured sciatic nerve. Both cellular senescence and proliferation were less vigorous in the dorsal root ganglia than in sciatic nerve stumps. These results reveal the dynamic changes of injury-induced cellular senescence and proliferation from both genetic and morphological aspects, and thus extend our understanding of the biological processes following peripheral nerve injury. The study was approved by the Animal Ethics Committee of Nantong University, China (approval No. 20190226-001) on February 26, 2019.

Key words: bioinformatic analysis, cellular senescence, dorsal root ganglia, p16, peripheral nerve regeneration, peripheral nerve trauma, proliferation, rat sciatic nerves, sciatic nerve crush, β-galactosidase activities