中国神经再生研究(英文版) ›› 2023, Vol. 18 ›› Issue (1): 176-182.doi: 10.4103/1673-5374.344839

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

弥散张量成像揭示犬脊髓损伤后脑结构的变化

  

  • 出版日期:2023-01-15 发布日期:2022-06-17
  • 基金资助:
    国家自然科学基金项目(82102676),北京市科委项目(Z171100001017076),国家重点研发计划项目(2018YFF0301104),诱导神经干细胞治疗军事脊髓损伤的医疗保护技术及应用研究项目(Z181100004118004)

Diffusion tensor imaging reveals brain structure changes in dogs after spinal cord injury

Chang-Bin Liu1, De-Gang Yang2, Jun Li2, Chuan Qin2, 3, Xin Zhang2, 3, Jun Liu2, 3, Da-Peng Li4, *, Jian-Jun Li2, 3, *   

  1. 1Department of Rehabilitation Medicine, Beijing Tiantan Hospital, Capital Medical University, Beijing, China; 2Department of Spinal and Neural Function Reconstruction, China Rehabilitation Research Center, Beijing, China; 3China Rehabilitation Science Institute, Beijing, China; 4Department of Neurosurgery, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, Beijing, China
  • Online:2023-01-15 Published:2022-06-17
  • Contact: Jian-Jun Li, master, 13718331416@163.com; Da-Peng Li, PhD, 15901518431@163.com.
  • Supported by:
    This work was supported by the National Natural Science Foundation of China, No. 82102676 (to CBL), a grant from Beijing Municipal Science & Technology Commission, No. Z171100001017076 (to JJL), National Key Research and Development Program, No. 2018YFF0301104 (to JJL) and Research on Medical Protection Technology and Application of Induced Neural Stem Cells in the Treatment of Military Spinal Cord Injury, No. Z181100004118004 (to JL).

摘要:

由于脊髓通路中的沃勒变性、突触连接的变化及其他可改变大脑细胞结构的脊髓相关细胞反应,因而推测脊髓损伤后大脑弥散张量成像参数可能会发生变化,但弥散张量成像参数随时间的动态变化仍有待研究。实验对大动物比格犬建立的T10脊髓挫伤模型进行弥散张量成像观察,结果发现,在损伤后12周内,随着损伤后时间的延长,犬大脑脚、内囊后肢、中央前回及中央后回脑区的弥散张量成像参数出现了明显的改变,并以免疫组化染色检测这些部位中轴突标志物神经丝重链多肽胶质细胞标志物胶质纤维酸性蛋白和神经元标志物NeuN阳性表达情况,其结果显示这些病理变化与弥散张量成像的变化的一致性。这一结果说明可以使用弥散张量成像观察脊髓损伤后出现的大脑结构的变化。

https://orcid.org/0000-0003-2663-5971 (Jian-Jun Li); https://orcid.org/0000-0002-4046-5510 (Da-Peng Li)

关键词: 脊髓损伤, 弥散张量成像, 犬, 病理生理学, 脑-脊髓结构, 皮质脊髓束, 磁共振成像, 各向异性分数, 表观弥散系数

Abstract: Based on the Wallerian degeneration in the spinal cord pathways, the changes in synaptic connections, and the spinal cord-related cellular responses that alter the cellular structure of the brain, we presumed that brain diffusion tensor imaging (DTI) parameters may change after spinal cord injury. However, the dynamic changes in DTI parameters remain unclear. We established a Beagle dog model of T10 spinal cord contusion and performed DTI of the injured spinal cord. We found dynamic changes in DTI parameters in the cerebral peduncle, posterior limb of the internal capsule, pre- and postcentral gyri of the brain within 12 weeks after spinal cord injury. We then performed immunohistochemistry to detect the expression of neurofilament heavy polypeptide (axonal marker), glial fibrillary acidic protein (glial cell marker), and NeuN (neuronal marker). We found that these pathological changes were consistent with DTI parameter changes. These findings suggest that DTI can display brain structure changes after spinal cord injury.

Key words: spinal cord injury, diffusion tensor imaging, canines, pathophysiology, cerebrospinal structures, corticospinal tract, magnetic resonance imaging, anisotropic fraction, apparent dispersion coefficient