中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2026, Vol. 21 ›› Issue (6): 2553-2562.doi: 10.4103/NRR.NRR-D-24-01165

• 原著:退行性病与再生 • 上一篇    下一篇

RBAD数据库：首个与阿尔茨海默病血液RNA改变及其临床关联性相关的数据库

  

  • 出版日期:2026-06-15 发布日期:2026-04-18
  • 基金资助:
    武汉亚洲总医院科研创新基金项目（2022KYCX1-B10）、湖北省自然科学基金项目（2023AFB550，2024AFB853）、国家自然科学基金项目（32400554，82371444）、湖北省教育厅科研计划指导项目（B2021016）

RBAD: The first database dedicated alterations of blood RNA in individuals with Alzheimer’s disease and their clinical relevance

Tingting Duan1, 2, 3, #, Jinyu Chu2, 3, #, Jinquan Li2, 3, Shiyao Pan2, 3, Dan Liu2, 3, Guirong Cheng2, 3, Yu Luo2, 3, Wen Zhou2, 3, Zhiming Wang2, 3, Wei Tan4, Qiong Wu2, 3, *, Yan Zeng1, 2, 3, 4, *, Feifei Hu1, 2, 3, 4, *   

  1. 1Department of Internal Medicine, Wuhan Asia General Hospital, Wuhan University of Science and Technology, Wuhan, Hubei Province, China; 
    2Brain Science and Advanced Technology Institute, School of Medicine, Wuhan University of Science and Technology, Wuhan, Hubei Province, China; 
    3School of Public Health, Wuhan University of Science and Technology, Wuhan, Hubei Province, China; 
    4Community Health Service Center, Geriatric Hospital, Wuhan University of Science and Technology, Wuhan, Hubei Province, China
  • Online:2026-06-15 Published:2026-04-18
  • Contact: Feifei Hu, PhD, hufeifei@wust.edu.cn; Yan Zeng, PhD, zengyan68@wust.edu.cn; Qiong Wu, PhD, qiongwu@wust.edu.cn.
  • Supported by:
    This study was supported by Research and Innovation Foundation of Wuhan Asia General Hospital, No. 2022KYCX1-B10 (to FH); the Natural Science Foundation of Hubei Province, No. 2023AFB550 (to FH); the National Natural Science Foundation of China, Nos. 32400554 (to FH), 82371444 (to YZ); the Guiding Project of the Scientific Research Program of the Department of Education of Hubei Province, No. B2021016 (to FH); and the Natural Science Foundation of Hubei Province, No. 2024AFB853 (to QW).

PDF

2

摘要：

脑组织中与阿尔茨海默病相关的转录组图谱已被描绘出来，然而，阿尔茨海默病患者血液RNA的变化及其临床相关性仍然不得而知。研究通过整合1468个人类和小鼠血液样本的 bulk RNA-seq、miRNA-seq和scRNA-seq数据，历经超过1100万次分析，获得了超过20000个血液特征，并基于这些分析结果构建了一个阿尔茨海默病血液RNA数据库（RBAD，http://www.bioinform.cn/RBAD/）。基于RBAD首先证实了阿尔茨海默病患者中嗅觉转导通路异常，同时可见红系细胞比例和功能下调，这可能是由于其CD45增加以及与GZMK+CD8+T细胞的相互作用。此外，还发现了449种与生存相关的血液RNA以及2种与认知衰退相关的mRNA（H4C3和CTU1）。研究首次构建了一个与阿尔茨海默病相关的血液RNA特征资源库，这将为探索阿尔茨海默病周围生物标志物和致病机制提供帮助。


https://orcid.org/0000-0002-8884-3568 (Feifei Hu); https://orcid.org/0000-0002-5199-5448 (Yan Zeng); 

https://orcid.org/0009-0009-2513-5343 (Qiong Wu)

关键词: 阿尔茨海默病, 轻度认知障碍, 血液, RNA, 生物标志物, 高通量测序, 临床相关性, 红系细胞, 嗅觉传导, GZMK+CD8+T细胞

Abstract: Alzheimer’s disease-associated transcriptomic landscapes have been defined in brain tissue. However, changes in blood RNA and their clinical relevance remain poorly understood. In this study, we developed an RNA profile based on 1468 blood samples from both human and mouse studies, which include bulk RNA sequencing (RNA-seq), microRNA-seq, and single-cell RNA-seq data. We developed a comprehensive analysis pipeline that conducted over 11 million comparisons and correlations to identify more than 20,000 blood features. With these findings, we established a blood RNA database related to Alzheimer’s disease, RNAs in Blood of AD (RBAD, http://www.bioinform.cn/RBAD/). Using RBAD, we initially validated well-established Alzheimer’s disease-related pathways, including olfactory transduction. We then observed a decrease in both the proportion and functionality of erythroid cells, likely attributed to their elevated CD45 levels and interactions with GZMK+ CD8+ T cells. Furthermore, we identified 449 blood RNAs linked to patients’ overall survival, along with two mRNAs (H4C3 and CTU1) associated with cognitive decline. In summary, RBAD is the first web-based analysis platform dedicated to investigating blood RNA changes in Alzheimer’s disease, and provides valuable insights into potential peripheral biomarkers and pathogenic mechanisms related to Alzheimer’s disease. 

Key words: Alzheimer’s disease, biomarker, blood, clinical relevance, erythroid cells, GZMK+ CD8+ T cells, high throughput sequencing, mild cognitive impairment, olfactory transduction, RNA