中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2026, Vol. 21 ›› Issue (6): 2658-2668.doi: 10.4103/NRR.NRR-D-24-01544

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

多功能神经导管PLGA/CNT-PDA-NT3修复损伤周围神经

  

  • 出版日期:2026-06-15 发布日期:2026-04-17
  • 基金资助:

    研究团队感谢国家重点研发计划、北京市自然科学基金、浙江省医药卫生技术规划项目、浙江省医药卫生技术规划项目、浙江省医药卫生技术规划项目、浙江省医药卫生科技规划项目、宁波市骨科与运动康复临床研究中心的支持。

Polydopamine-coupled NT3-derived oriented conductive scaffolds with immunomodulatory properties accelerate peripheral nerve regeneration

Xiaokun Chen1, #, Jihai Xu2, #, Ziyuan Yang3, 4, 5, #, Jiahua Zhou3, 4, 6, Feng Qin3, 4, 6, Xueyuan Li7, Miao Yu7, Yanhua Wang3, 4, 6, *, Ming Li3, 4, 5, *, Xin Wang8, *   

  1. 1Department of Orthopedic Surgery, Ninth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China; 
    2Department of Hand Surgery, Department of Plastic Reconstructive Surgery, Ningbo No.6 Hospital, Ningbo, Zhejiang Province, China; 
    3Key Laboratory of Trauma and Neural Regeneration, Ministry of Education, Peking University, Beijing, China; 
    4National Center for Trauma Medicine, Beijing, China; 
    5Trauma Medicine Center, Peking University People’s Hospital, Beijing, China; 
    6Department of Orthopedics and Trauma, Peking University People’s Hospital, Beijing, China; 
    7Department of Hand Surgery, Ningbo No.6 Hospital, Ningbo, Zhejiang Province, China; 
    8Department of Plastic Reconstructive Surgery, Ningbo No.6 Hospital, Ningbo, Zhejiang Province, China
  • Online:2026-06-15 Published:2026-04-17
  • Contact: Yanhua Wang, PhD, 94719599@qq.com; Ming Li, PhD, liming_ort@bjmu.edu.cn; Xin Wang, MD, dr.wangxin@qq.com.
  • Supported by:
    This study was supported by the National Key R&D Program of China, No. 2022YFC3006200 (to YW); the Natural Science Foundation of Beijing, No. 7232190 (to YW); Zhejiang Province Medical and Health Technology Plan Project, Nos. 2022020506 (to XW), 2024KY1612 (to JX), 2024KY1615 (to MY); and Ningbo Clinical Research Center for Orthopedics and Sports Rehabilitation, No. 2024L004 (to XW).

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摘要：

周围神经损伤是一种复杂的疾病，由于周围神经再生能力有限，临床治疗面临重大挑战。神经导管被视为克服其他治疗方法（如神经移植）不足的有力策略。然而，神经再生发生在复杂的环境中，需要精细的调控以满足修复需求。实验旨在探索一种多功能神经导管，其具备清除活性氧、免疫调节以重塑再生环境，以及通过拓扑提示和电信号引导神经生长的功能。实验采用电纺技术制备了具有定向结构的电活性神经引导导管，并采用贻贝启发的聚多巴胺涂层结合神经营养因子3进行修饰。所得神经支架展现出良好的定向性、电导率和力学性能。神经导管中神经营养因子3的持续释放支持了修复过程中的神经再生。体外评估证实了神经支架的细胞相容性、活性氧清除能力和免疫调节功能。在大鼠坐骨神经缺损模型中，神经支架在12周内有效防止了肌肉萎缩，并促进了神经再生和功能恢复。这些结果表明，具有多种生物活性功能的聚多巴胺修饰电活性定向神经导管在损伤周围神经修复中具有潜力。


https://orcid.org/0000-0001-5121-7750 (Ming Li)

关键词: 碳纳米管, 电纺神经导管, 免疫调节, 神经营养因子3, 周围神经再生

Abstract: Peripheral nerve injury is a complex condition presenting significant clinical treatment challenges due to the limited regenerative capacity of peripheral nerves. Nerve conduits have been seen as a promising strategy to overcome the shortage of other treatment options (e.g., nerve graft). However, nerve regeneration occurs within a complex environment, and elaborate modulation is needed to meet repair requirements. The aim of this study was to investigate and explore a multifunctional nerve conduit with reactive oxygen species clearing, immune modulation to reshape the regenerative environment, and topographic cues and electrical signals to guide nerve growth. We developed an electroactive nerve guidance conduit composed of polylactic-glycolic acid and carbon nanotubes with an oriented structure using electrospinning and modified it with mussel-inspired polydopamine combining neurotrophin-3. The resulting nerve scaffold exhibited favorable orientation, electrical conductivity, and mechanical properties. Continuous release of neurotrophin-3 from the nerve conduit supported nerve regeneration throughout the repair process. In vitro assessments confirmed the cytocompatibility, reactive oxygen species scavenging, and immune regulation capabilities of the nerve scaffolds. In a rat sciatic nerve defect model, the nerve scaffolds effectively prevented muscle atrophy and promoted nerve regeneration and functional recovery over a 12-week period. These findings suggest that polydopamine-modified, electroactive, oriented nerve guidance conduits with multiple bioactive functions hold great promise for the repair of peripheral nerve injuries.

Key words: carbon nanotubes, electrospinning nerve catheter, immune regulation, neurotrophin-3, peripheral nerve regeneration