中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2020, Vol. 15 ›› Issue (11): 2108-2115.doi: 10.4103/1673-5374.282270

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

术中单次应用中性粒细胞肽1可以加速挤压伤后周围神经的早期功能恢复

  

  • 出版日期:2020-11-15 发布日期:2020-08-23
  • 基金资助:

    国家自然科学基金（31571236）；中国教育部创伤与神经再生重点实验室（北京大学）项目（BMU2019XY007-01）；中国教育部创新计划（IRT R01）；国家自然科学基金研究开发基金北京大学人民医院项目（RDH2017-01，RDY2018-09）。

Intraoperative single administration of neutrophil peptide 1 accelerates the early functional recovery of peripheral nerves after crush injury

Yu-Song Yuan1, 2, Su-Ping Niu3, Fei Yu1, 4 , Ya-Jun Zhang5 , Na Han2, 3, Hao Lu1, 6 , Xiao-Feng Yin1, 2 , Hai-Lin Xu1, 6, Yu-Hui Kou1, 2   

  1. 1 Department of Trauma and Orthopedics, Peking University People’s Hospital, Peking University, Beijing, China
    2 Key Laboratory of Trauma and Neural Regeneration (Peking University), Ministry of Education, Beijing, China
    3 Office of Academic Research, Peking University People’s Hospital, Beijing, China
    4 National and Local Joint Engineering Research Center of Orthopaedic Biomaterials, Department of Bone & Joint Surgery, Peking University Shenzhen Hospital, Shenzhen, Guangdong Province, China
    5 National Center for Trauma Medicine, Beijing, China
    6 Diabetic Foot Treatment Center, Peking University People’s Hospital, Peking University, Beijing, China
  • Online:2020-11-15 Published:2020-08-23
  • Contact: Hai-Lin Xu, MD, xuhailinfa@163.com; Yu-Hui Kou, PhD,yuhuikou@bjmu.edu.cn.
  • Supported by:
    This research was funded by the National Natural Science Foundation of China, No. 31571236 (to YHK); the Key Laboratory of Trauma and Neural Regeneration (Peking University), Ministry of Education, China, No. BMU2019XY007-01 (to YHK); the Ministry of Education Innovation Program of China, No. IRT_16R01 (to YHK); the Research and Development Funds of Peking University People’s Hospital, China, Nos. RDH2017-01 (to YHK), RDY2018-09 (to HL).

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摘要：

中性粒细胞肽1(NP-1)是一类参与固有免疫的多肽，连续肌肉注射能够促进周围神经的再生，但是由于操作过程复杂，不利于临床应用。为此，实验观察中性粒细胞肽1术中单次给药对周围神经再生的作用。采用钳夹法建立坐骨神经挤压伤大鼠模型，建模后盐水组和中性粒细胞肽1组分别于术中将生理盐水或10 µg/mL的中性粒细胞肽1单次注射在大鼠坐骨神经所在的肌间隙中。(1)坐骨神经功能检测、神经电生理检测及苏木精-伊红染色显示：术后4周，中性粒细胞肽1组神经传导速度、坐骨神经功能指数及胫前肌肌纤维横截面积均高于盐水组，术后8周，两组上述指标间则无显著差异；(2)胫前肌湿质量检测和坐骨神组织学染色显示：术后4，8周，中性粒细胞肽1组和盐水组之间胫前肌湿质量、坐骨神经有髓神经纤维数量和轴突的横截面积无差异；(3)上述数据提供：术中单次应用中性粒细胞肽1在坐骨神经损伤后4周可发挥其促进神经功能恢复的作用。

orcid: 0000-0003-1829-3852 (Hai-Lin Xu) 

         0000-0003-1523-825X (Yu-Hui Kou)

关键词: 挤压伤, 防御素1, 中性粒细胞肽1, 步态分析, 术中管理, 神经传导速度, 周围神经损伤, 周围神经再生, 坐骨神经, 胫前肌, 创伤, 神经系统

Abstract: Neutrophil peptide 1 belongs to a family of peptides involved in innate immunity. Continuous intramuscular injection of neutrophil pep- tide 1 can promote the regeneration of peripheral nerves, but clinical application in this manner is not convenient. To this end, the effects of a single intraoperative administration of neutrophil peptide 1 on peripheral nerve regeneration were experimentally observed. A rat model of sciatic nerve crush injury was established using the clamp method. After model establishment, a normal saline group and a neu- trophil peptide 1 group were injected with a single dose of normal saline or 10 µg/mL neutrophil peptide 1, respectively. A sham group, without sciatic nerve crush was also prepared as a control. Sciatic nerve function tests, neuroelectrophysiological tests, and hematoxy- lin-eosin staining showed that the nerve conduction velocity, sciatic functional index, and tibialis anterior muscle fiber cross-sectional area were better in the neutrophil peptide 1 group than in the normal saline group at 4 weeks after surgery. At 4 and 8 weeks after surgery, there were no differences in the wet weight of the tibialis anterior muscle between the neutrophil peptide 1 and saline groups. Histological stain- ing of the sciatic nerve showed no significant differences in the number of myelinated nerve fibers or the axon cross-sectional area between the neutrophil peptide 1 and normal saline groups. The above data confirmed that a single dose of neutrophil peptide 1 during surgery can promote the recovery of neurological function 4 weeks after sciatic nerve injury. All the experiments were approved by the Medical Ethics Committee of Peking University People’s Hospital, China (approval No. 2015-50) on December 9, 2015.

Key words: crush injury, defensin 1, gait analysis, intraoperative administration, nerve conduction velocity, nervous system, neutrophil peptide 1, peripheral nerve injury, peripheral nerve regeneration, sciatic nerve, tibialis anterior muscle, trauma