中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (20): 1839-1843.doi: 10.4103/1673-5374.143432

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

臂丛神经撕脱伤致阴茎勃起功能障碍大鼠模型的建立

  

  • 收稿日期:2013-11-30 出版日期:2014-10-25 发布日期:2014-10-25
  • 基金资助:

    国家重点临床专家建设项目；广东省科技计划项目

Penile erectile dysfunction after brachial plexus root avulsion injury in rats

Guo Fu 1, Bengang Qin 1, Li Jiang 2, Xijun Huang 3, Qinsen Lu 4, Dechun Zhang 5, Xiaolin Liu1, Jiakai Zhu 1, Jianwen Zheng 1, Xuejia Li 6, Liqiang Gu 1   

  1. 1 Department of Orthopedic Trauma & Microsurgery, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong Province, China
    2 Orthopedic Institute, Eastern Hospital, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong Province, China
    3 Department of Upper Limb Orthopedics, Eastern Hospital, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong
    Province, China
    4 Emergency Department, Shandong Provincial Hospital, Jinan, Shandong Province, China
    5 Department of Orthopedics, the Affiliated Hospital of Binzhou Medical College, Binzhou, Shandong Province, China
    6 Office of Research Service, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong Province, China
  • Received:2013-11-30 Online:2014-10-25 Published:2014-10-25
  • Contact: Liqiang Gu, Department of Microsurgery &Orthopedic Trauma Surgery, the First Affiliated Hospital of Sun Yat-sen University, 58 Zhongshan 2nd Road, Guangzhou 510080, Guangdong Province, China, guliqiang1963@aliyun.com.
  • Supported by:

    This study was supported by the National Key Clinical Specialist Construction Programs of China, No. 201402016 and the Science and Technology Planning Project of Guangdong Province, China, No. 2011A032100001.

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摘要：

作者既往研究发现，臂丛神经根撕脱伤男性患者常会表现出不同程度的阴茎勃起功能障碍，但发生机制尚不明确。实验通过颈椎后路手术建立了臂丛神经根撕脱伤大鼠模型，同时在操作时，致脊髓白质流出，建立合并脊髓损伤大鼠模型。颈部皮下注射阿朴吗啡以观察阴茎勃起功能。发现未合并/合并脊髓损伤的臂丛神经根撕脱伤大鼠阴茎勃起次数均明显减少。单纯臂丛神经根撕脱伤大鼠阴茎神经元型一氧化氮合酶表达无明显变化，合并脊髓损伤的大鼠阴茎神经元型一氧化氮合酶表达明显下降。说明阴茎神经元型一氧化氮合酶表达下降可能是合并脊髓损伤的臂丛神经根撕脱伤后阴茎勃起功能障碍的机制。

关键词: 神经再生, 臂丛神经根撕脱伤, 脊髓损伤, 周围神经损伤, 阴茎, 臂丛神经, 神经元型一氧化氮合酶, 勃起功能障碍, 大鼠模型

Abstract:

Our previous studies have demonstrated that some male patients suffering from brachial plexus injury, particularly brachial plexus root avulsion, show erectile dysfunction to varying degrees. However, the underlying mechanism remains poorly understood. In this study, we evaluated the erectile function after establishing brachial plexus root avulsion models with or without spinal cord injury  in rats. After these models were established, we administered apomorphine (via a subcutaneous injection in the neck) to observe changes in erectile function. Rats subjected to simple brachial plexus root avulsion or those subjected to brachial plexus root avulsion combined with spinal cord injury had significantly fewer erections than those subjected to the sham operation. Expression of neuronal nitric oxide synthase did not change in brachial plexus root avulsion rats. However, neuronal nitric oxide synthase expression was significantly decreased in brachial plexus root avulsion + spinal cord injury rats. These findings suggest that a decrease in neuronal nitric oxide synthase expression in the penis may play a role in erectile dysfunction caused by the combination of brachial plexus root avulsion and spinal cord injury.

Key words: nerve regeneration, brachial plexus avulsion, spinal cord injury, peripheral nerve injury, penis, neuronal nitric oxide synthase, erectile dysfunction, rat model, neural regeneration