中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (24): 2159-2163.doi: 10.4103/1673-5374.147947

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

人羊膜上皮细胞移植修复损伤臂丛神经:病理及生物力学分析

  

  • 收稿日期:2014-10-20 出版日期:2014-12-25 发布日期:2014-12-25

Transplantation of human amniotic epithelial cells repairs brachial plexus injury: pathological and biomechanical analyses

Qi Yang 1, Min Luo 1, Peng Li 2, Hai Jin 1     

  1. 1 China-Japan Union Hospital of Jilin University, Changchun, Jilin Province, China
    2 Department of Mechanics, School of Mechanical Science and Engineering, Jilin University, Changchun, Jilin Province, China
  • Received:2014-10-20 Online:2014-12-25 Published:2014-12-25
  • Contact: Hai Jin, M.D., China-Japan Union Hospital of Jilin University, Changchun 130033, Jilin Province, China, plyangqi1980@sina.com.
  • Supported by:

    This study was financially supported by a grant from the Science and Technology Development Project of Jilin Province of China, No. 20110492.

摘要:

以牵拉神经根的方法制备C6臂丛神经损伤兔模型,造模后即刻将人羊膜上皮细胞悬液注入到兔臂丛神经损伤区,24周后臂丛神经拉伸实验发现,其拉伸弹性限度应变、弹性限度应力、最大应力、最大应变明显增大,同时HE染色也证实臂丛神经病理形态明显改善,且兔前爪功能也明显恢复。作者认为,该结果说明人羊膜上皮细胞能对损伤臂丛神经产生修复效果,具有临床转化医学应用的潜力。

关键词: 神经再生, 周围神经损伤, 臂丛神经损伤, 动物模型, 人羊膜上皮细胞, 前爪功能, 组织形态, 拉伸力学

Abstract:

A brachial plexus injury model was established in rabbits by stretching the C6 nerve root. Immediately after the stretching, a suspension of human amniotic epithelial cells was injected into the injured brachial plexus. The results of tensile mechanical testing of the brachial plexus showed that the tensile elastic limit strain, elastic limit stress, maximum stress, and maximum strain of the injured brachial plexuses were significantly increased at 24 weeks after the injection. The treatment clearly improved the pathological morphology of the injured brachial plexus nerve, as seen by hematoxylin eosin staining, and the functions of the rabbit forepaw were restored. These data indicate that the injection of human amniotic epithelial cells contributed to the repair of brachial plexus injury, and that this technique may transform into current clinical treatment strategies.

Key words: nerve regeneration, peripheral nerve injury, brachial plexus injury, animal model, human amniotic epithelial cells, forepaw function, morphology, tensile mechanics, neural regeneration