中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (2): 230-236.doi: 10.4103/1673-5374.152376

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

依达拉奉联合许旺细胞移植能够修复大鼠脊髓损伤？

  

  • 收稿日期:2014-10-13 出版日期:2015-02-17 发布日期:2015-02-17

Edaravone combined with Schwann cell transplantation may repair spinal cord injury in rats

Shu-quan Zhang 1, Min-fei Wu  2, Zhe Piao 1, Jin Yao 1, Ji-hai Li 1, Xin-gang Wang 3, Jun Liu 4   

  1. 1 Department of Orthopedics, Tianjin Nankai Hospital, Nankai, Tianjin, China
    2 Department of Spine Surgery, Orthopedic Hospital, Second Hospital, Clinical Hospital, Jilin University, Changchun, Jilin Province, China
    3 Department of Burns and Plastic Surgery, China-Japan Union Hospital, Jilin University, Changchun, Jilin Province, China
    4 Department of Hand and Foot Surgery and Reparative & Reconstructive Surgery Center, Orthopedic Hospital, Second Hospital, Clinical
    Hospital, Jilin University, Changchun, Jilin Province, China
  • Received:2014-10-13 Online:2015-02-17 Published:2015-02-17
  • Contact: Jun Liu, zjianj1a1@126.com

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摘要：

已知依达拉奉可延缓神经细胞凋亡，改善神经功能且可改善脊髓损伤区微环境，为SC移植治疗脊髓损伤提供有利环境。为此，实验建立了胸9脊髓全横断损伤模型大鼠，6h后于损伤处头尾两端植入许旺细胞，同时经尾静脉注射依达拉奉。8周后PKH-26标记的许旺细胞在依达拉奉联合许旺细胞治疗的大鼠体内存活并向脊髓损伤中心迁移，且大鼠脊髓组织中PKH-26标记的许旺细胞的数量比单纯许旺细胞移植治疗或不治疗的大鼠多，同时辣根过氧化物酶逆行神经示踪脊髓追踪显示依达拉奉联合许旺细胞治疗的大鼠脊髓组织中辣根过氧化物酶阳性神经纤维数量也比单纯许旺细胞移植治疗的大鼠多。实验结果显示，依达拉奉联合许旺细胞治疗的大鼠下肢运动功能和神经电生理功能优于单纯许旺细胞移植治疗的大鼠，表明许旺细胞移植联合依达拉奉干预可促进脊髓损伤大鼠神经纤维的再生，改善其神经功能。
 

关键词: 神经再生, 脊髓损伤, 许旺细胞, 细胞移植, 依达拉奉, 运动功能, 电生理功能

Abstract:

Edaravone has been shown to delay neuronal apoptosis, thereby improving nerve function and the microenvironment after spinal cord injury. Edaravone can provide a favorable environment for the treatment of spinal cord injury using Schwann cell transplantation. This study used rat models of complete spinal cord transection at T9. Six hours later, Schwann cells were transplanted in the head and tail ends of the injury site. Simultaneously, edaravone was injected through the caudal vein. Eight weeks later, the PKH-26-labeled Schwann cells had survived and migrated to the center of the spinal cord injury region in rats after combined treatment with edaravone and Schwann cells. Moreover, the number of PKH-26-labeled Schwann cells in the rat spinal cord was more than that in rats undergoing Schwann cell transplantation alone or rats without any treatment. Horseradish peroxidase retrograde tracing revealed that the number of horseradish peroxidase-positive nerve fibers was greater in rats treated with edaravone combined with  Schwann cells than in rats with Schwann cell transplantation alone. The results demonstrated that lower extremity motor function and neurophysiological function were better in rats treated with edaravone and Schwann cells than in rats with Schwann cell transplantation only. These data confirmed that Schwann cell transplantation combined with edaravone injection promoted the regeneration of nerve fibers of rats with spinal cord injury and improved neurological function.

Key words: nerve regeneration, spinal cord injury, Schwann cells, cell transplantation, edaravone, motor function, electrophysiological function, neural regeneration