中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (2): 286-291.doi: 10.4103/1673-5374.152384

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

安宫牛黄贴纸穴位敷贴保护脑缺血模型大鼠海马和皮质神经元

  

  • 收稿日期:2014-07-02 出版日期:2015-02-17 发布日期:2015-02-17

Point application with Angong Niuhuang sticker protects hippocampal and cortical neurons in rats with cerebral ischemia

Dong-shu Zhang, Yuan-liang Liu, Dao-qi Zhu, Xiao-jing Huang, Chao-hua Luo   

  1. College of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong Province, China
  • Received:2014-07-02 Online:2015-02-17 Published:2015-02-17
  • Contact: Dong-shu Zhang, M.D., nyzds@sina.com.

摘要:

传统中药制剂安宫牛黄丸能改善急性缺血性卒中的神经功能,但因其使用不便和药物含有毒性成分朱砂和雄黄,已改制剂型加入透皮吸收促进剂制成贴纸,其安全剂量范围及临床适应证都有所扩大。实验采用1.35,2.7,5.4g/kg剂量的安宫牛黄贴纸对慢性脑缺血模型大鼠的大椎(DU14)、气海(RN6)和命门(DU4)进行穴位敷贴4周。以Morris水迷宫和病理观察结果验证其效果。实验结果显示中、高剂量安宫牛黄贴纸穴位敷贴能有效减少大鼠大脑海马CA1区和大脑皮质的神经元损伤,改善慢性脑缺血大鼠的记忆能力,且效果接近于电针大椎、气海和命门的干预结果。说明安宫牛黄贴纸穴位敷贴能有效保护慢性脑缺血,且疗效与针灸等同。

关键词: 神经再生, 脑缺血, 穴位敷贴, 安宫牛黄贴纸, 脑损伤, 神经功能, 针灸, 中医药

Abstract:

Angong Niuhuang pill, a Chinese materia medica preparation, can improve neurological functions after acute ischemic stroke. Because of its inconvenient application and toxic components (Cinnabaris and Realgar), we used transdermal enhancers to deliver Angong Niuhuang pill by modern technology, which expanded the safe dose range and clinical indications. In this study, Angong Niuhuang stickers administered at different point application doses (1.35, 2.7, and
5.4 g/kg) were administered to the Dazhui (DU14), Qihai (RN6) and Mingmen (DU4) of rats with chronic cerebral ischemia, for 4 weeks. The Morris water maze was used to determine the learning and memory ability of rats. Hematoxylin-eosin staining and Nissl staining were used to observe neuronal damage of the cortex and hippocampal CA1 region in rats with chronic cerebral ischemia. The middle- and high-dose point application of Angong Niuhuang stickers attenuated neuronal damage in the cortex and hippocampal CA1 region, and improved the memory of rats with chronic cerebral ischemia with an efficacy similar to interventions by electroacupuncture at Dazhui (DU14), Qihai (RN6) and Mingmen (DU4). Our experimental findings indicate that point application with Angong Niuhuang stickers can improve cognitive function after chronic cerebral ischemia in rats and is neuroprotective with an equivalent efficacy to acupuncture.

Key words: nerve regeneration, cerebral ischemia, point application, Angong Niuhuang sticker, brain injury, neurological functions, acupuncture, traditional Chinese medicine, NSFC grants, neural regeneration