中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (3): 457-462.doi: 10.4103/1673-5374.153696

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

针刺抑制脑出血大鼠基底节Notch1及Hes1蛋白表达的保护机制

  

  • 收稿日期:2014-11-12 出版日期:2015-03-20 发布日期:2015-03-20
  • 基金资助:

    国家自然科学基金（81273824）

Acupuncture inhibits Notch1 and Hes1 protein expression in the basal ganglia of rats with cerebral hemorrhage

Wei Zou 1, Qiu-xin Chen 2, Xiao-wei Sun 1, Qing-bin Chi 1, Hong-yu Kuang 3, Xue-ping Yu 1, Xiao-hong Dai 1   

  1. 1 Third Department of Acupuncture, First Affiliated Hospital, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang Province, China
    2 Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang Province, China
    3 Department of Endocrinology, First Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang Province, China
  • Received:2014-11-12 Online:2015-03-20 Published:2015-03-20
  • Contact: Wei Zou, kuangzou2013@sina.com
  • Supported by:

    This study was supported by the National Natural Science Foundation of China, No. 81273824, 30772840; Ministry of Education Doctoral Fund in China, No. 20102327110003; the Natural Science Foundation of Heilongjiang Province in China, No. ZD201204; and Special funds for Technological Innovation Research of Harbin, China, No. 2012RFXXS062

PDF

850

摘要：

Notch信号途径的激活能够维持神经干细胞的自我增殖状态，提高神经修复能力。目前对脑出血损伤信号传导通路变化的研究较少。我们采用针刺干预脑出血模型大鼠，实验分为4组：空白组不做任何干预；模型组采用自体血脑内注入法制作脑出血模型大鼠；针刺组在模型组基础上给予针刺大鼠百会穴(DU 20)曲鬓穴(GB 7)干预，每日1次，30 min/次；DAPT组在模型组干预基础上再将0.15 ?g/?L DAPT溶液10 ?L注入脑内。以免疫组织化学及免疫印迹检测结果发现，针刺能够有效的抑制大鼠基底节组织中Notch1及Hes1蛋白的表达，与Notch信号通路的抑制剂DAPT作用后效果相当。说明针刺可能通过抑制脑出血大鼠基底节Notch-Hes信号通路的传导，对脑出血起神经保护作用。

关键词: 神经再生, 针灸, 脑出血, Notch1, Hes1, 大鼠, DAPT, 免疫组织化学, 免疫印迹, 神经再生, 神经干细胞, 国家自然科学基金

Abstract:

Notch pathway activation maintains neural stem cells in a proliferating state and increases nerve repair capacity. To date, studies have rarely focused on changes or damage to signal transduction pathways during cerebral hemorrhage. Here, we examined the effect of acupuncture in a rat model of cerebral hemorrhage. We examined four groups: in the control group, rats received no treatment. In the model group, cerebral hemorrhage models were established by infusing non-heparinized blood into the brain. In the acupuncture group, modeled rats had Baihui (DU20) and Qubin (GB7) acupoints treated once a day for 30 minutes. In the DAPT group, modeled rats had 0.15 µg/mL DAPT solution (10 mL) infused into the brain. Immunohistochemistry and western blot results showed that acupuncture effectively inhibits Notch1 and Hes1 protein expression in rat basal ganglia. These inhibitory effects were identical to DAPT, a Notch signaling pathway inhibitor. Our results suggest that acupuncture has a neuroprotective effect on cerebral hemorrhage by inhibiting Notch-Hes signaling pathway transduction in rat basal ganglia after cerebral hemorrhage

Key words: nerve regeneration, acupuncture, cerebral hemorrhage, immunohistochemistry, western blot assay, Notch1, Hes1, rats, DAPT, neural stem cells, NSFC grant, neural regeneration