中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (10): 1656-1662.doi: 10.4103/1673-5374.167766

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

周围神经病理性疼痛干预的新靶点:氨酰tRNA合成酶

  

  • 收稿日期:2015-08-12 出版日期:2015-10-28 发布日期:2015-10-28
  • 基金资助:

    韩国科学、ICT、未来计划部国家研究基金

A novel therapeutic target for peripheral nerve injury-related diseases: aminoacyl-tRNA synthetases

Byung Sun Park1, Seung Geun Yeo2, Junyang Jung1, *, Na Young Jeong3, *   

  1. 1 Department of Anatomy and Neurobiology, School of Medicine, Kyung Hee University, Seoul, Republic of Korea
    2 Department of Otolaryngolgy, Head and Neck Surgery, School of Medicine, Kyung Hee University, Seoul, Republic of Korea
    3 Department of Anatomy and Cell Biology, College of Medicine, Dong-A University, Busan, Republic of Korea
  • Received:2015-08-12 Online:2015-10-28 Published:2015-10-28
  • Contact: Junyang Jung, M.D., Ph.D. or Na Young Jeong, M.D., Ph.D., jjung@khu.ac.kr or jnyjjy@dau.ac.kr.
  • Supported by:

    This study was supported by Basic Science Research Program through the National Research
    Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (2015R1A2A2A01002735 to JJ; 2015R1C1A1A02036863 to NYJ).

摘要:

氨酰tRNA合成酶除了是蛋白合成的关键酶外,还有一些非经典功能,如细胞周期调控和信号转导。因此,若这些非经典功能可被调控,氨酰tRNA合成酶将可成为有力的药物干预靶点。应用氨酰tRNA合成酶特异引物检测发现,坐骨神经切断后脊髓背角氨酰tRNA合成酶中的苯丙氨酸tRNA合成酶beta链,Isoleucyl-tRNA合成酶和甲硫氨酰tRNA合成酶mRNA表达在脊髓背角损伤侧神经元中明显升高,但在胶质细胞中则无此变化。由此表明,苯丙氨酸tRNA合成酶beta链,Isoleucyl-tRNA合成酶和甲硫氨酰tRNA合成酶可能传递了周围神经损伤后的异常感觉信号,并可能成为药物干预的新靶点。

关键词: 神经再生, 氨酰tRNA合成酶, 背角, 周围神经损伤, 原位杂交, 感觉

Abstract:

Aminoacyl-tRNA synthetases (AminoARSs) are essential enzymes that perform the first step of protein synthesis. Beyond their original roles, AminoARSs possess non-canonical functions, such as cell cycle regulation and signal transduction. Therefore, AminoARSs represent a powerful pharmaceutical target if their non-canonical functions can be controlled. Using AminoARSs-specific primers, we screened mRNA expression in the spinal cord dorsal horn of rats with peripheral nerve injury created by sciatic nerve axotomy. Of 20 AminoARSs, we found that phenylalanyl-tRNA synthetase beta chain (FARSB), isoleucyl-tRNA synthetase (IARS) and methionyl-tRNA synthetase (MARS) mRNA expression was increased in spinal dorsal horn neurons on the injured side, but not in glial cells. These findings suggest the possibility that FARSB, IARS and MARS, as a neurotransmitter, may transfer abnormal sensory signals after peripheral nerve damage and become a new target for drug treatment.

Key words: nerve regeneration, aminoacyl-tRNA synthetases, dorsal horn, peripheral nerve injury, in situ hybridization, neural regeneration