中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (4): 604-609.doi: 10.4103/1673-5374.180745

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

电针百会穴(DU20)上调宫内窘迫新生鼠脑组织神经源性分化因子mRNA的表达

  

  • 收稿日期:2016-03-07 出版日期:2016-04-30 发布日期:2016-04-30
  • 基金资助:

    福建省自然科学基金(2015J01133)、福建医科大学教授学术发展基金(JS11003)

Electroacupuncture at Baihui (DU20) acupoint up-regulates mRNA expression of NeuroD molecules in the brains of newborn rats suffering in utero fetal distress

Lu Chen, Yan Liu, Qiao-mei Lin, Lan Xue, Wei Wang, Jian-wen Xu*   

  1. Neurobiology Research Center, Department of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian Province, China
  • Received:2016-03-07 Online:2016-04-30 Published:2016-04-30
  • Contact: Jian-wen Xu, M.D., fjxjw@126.com.
  • Supported by:

    This research was supported by the Natural Science Foundation of Fujian Province of China, No. 2015J01133; the Professor Academic Development Foundation of Fujian Medical University of China, No. JS11003.

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摘要：

研究表明，神经源性分化因子在调控神经干细胞分化为成熟神经元的脑内微环境过程中发挥重要作用。为此，我们假设电针宫内窘迫新生鼠百会穴(DU20)，可调节神经源性分化因子的表达。实验将宫内窘迫20 min的新生SD大鼠，从3日龄(Day3)开始进行为期7 d的电针，以频率为2-10 Hz的疏密波电针百会穴干预，电针2周。实时荧光定量PCR检测发现，新生鼠脑组织神经源性分化因子mRNA表达随治疗结束后时间的延长呈现上升趋势，Day22达到最高峰，Day35表达水平与Day16相近，证实针百会穴(DU20)可有效促进宫内窘迫新生鼠脑组织神经源性分化因子mRNA的表达。

orcid: 0000-0001-9485-5402 (Jian-wen Xu)

关键词: 再生, 脑损伤, 胎儿宫内窘迫, 缺氧缺血性脑损伤, SD大鼠, 电针, 神经源性分化因子, 实时荧光定量PCR, 神经修复, 神经再生, 百会穴, 非穴

Abstract:

NeuroD plays a key regulatory effect on differentiation of neural stem cells into mature neurons in the brain. Thus, we assumed that
electroacupuncture at Baihui (DU20) acupoint in newborn rats exposed to in utero fetal distress would influence expression of NeuroD.
Electroacupuncture at Baihui was performed for 20 minutes on 3-day-old (Day 3) newborn Sprague-Dawley rats exposed to in utero fetal
distress; electroacupuncture parameters consisted of sparse and dense waves at a frequency of 2–10 Hz. Real-time fluorescent quantitative
PCR results demonstrated that mRNA expression of NeuroD, a molecule that indicates NeuroD, increased with prolonged time in brains
of newborn rats, and peaked on Day 22. The level of mRNA expression was similar between Day 16 and Day 35. These findings suggest that
electro acupuncture at Baihui acupoint could effectively increase mRNA expression of molecules involved in NeuroD in the brains of newborn
rats exposed to in utero fetal distress.

Key words: nerve regeneration, brain injury, in utero fetal distress, hypoxic-ischemic brain injury, electroacupuncture, real-time fluorescent quantitative PCR, NeuroD, nerve repair, Baihui (DU20) acupoint, non-acupoint, neural regeneration