关键词: 神经再生, 周围神经再生, 骨髓间充质干细胞, 细胞外基质, 许旺细胞, 背根神经节神经元, 生态位, 神经营养因子, 神经再生, 胶原, 层粘连蛋白, 纤维连接蛋白

Abstract: The extracellular matrix, which includes collagens, laminin, or fibronectin, plays an important role in peripheral nerve regeneration. Recently, a Schwann cell-derived extracellular matrix with classical biomaterial was used to mimic the neural niche. However, extensive clinical use of Schwann cells remains limited because of the limited origin, loss of an autologous nerve, and extended in vitro culture times. In the present study, human umbilical cord-derived mesenchymal stem cells (hUCMSCs), which are easily accessible and more proliferative than Schwann cells, were used to prepare an extracellular matrix. We identified the morphology and function of hUCMSCs and investigated their effect on peripheral nerve regeneration. Compared with a non-coated dish tissue culture, the hUCMSC-derived extracellular matrix enhanced Schwann cell proliferation, upregulated gene and protein expression levels of brain-derived neurotrophic factor, glial cell-derived neurotrophic factor, and vascular endothelial growth factor in Schwann cells, and enhanced neurite outgrowth from dorsal root ganglion neurons. These findings suggest that the hUCMSC-derived extracellular matrix promotes peripheral nerve repair and can be used as a basis for the rational design of engineered neural niches.

Key words: nerve regeneration, peripheral nerve regeneration, mesenchymal stem cells, extracellular matrix, Schwann cell, dorsal root ganglion neuron, niche, neurotropic factors, collagen, laminin, fibronectin, neural regeneration