中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2017, Vol. 12 ›› Issue (6): 987-994.doi: 10.4103/1673-5374.208595

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

脑损伤联合他克莫司促进损伤周围神经的修复

  

  • 收稿日期:2017-04-14 出版日期:2017-06-15 发布日期:2017-06-15
  • 基金资助:

     

    河北省卫生厅项目（20130027）；河北省科技厅项目（142777105D）；河北省承德市科技局项目（20123128）

Brain injury in combination with tacrolimus promotes the regeneration of injured peripheral nerves

Xin-ze He1, 5, Jian-jun Ma2, Hao-qi Wang2, Tie-min Hu3, Bo Sun1, Yun-feng Gao1, Shi-bo Liu1, Wei Wang4, Pei Wang1   

  1. 1 Department of Hand and Foot Surgery, Affiliated Hospital of Chengde Medical College, Chengde, Hebei Province, China; 2 Postgraduate School, Chengde Medical College, Chengde, Hebei Province, China; 3 Department of Neurosurgery, Affiliated Hospital of Chengde Medical College, Chengde, Hebei Province, China; 4 Department of Hand and Foot Surgery, the First Hospital of Qinhuangdao, Qinhuangdao, Hebei Province, China; 5 Binzhou Central Hospital, Binzhou, Shandong Province, China
  • Received:2017-04-14 Online:2017-06-15 Published:2017-06-15
  • Contact: Pei Wang, cdgkwp@sina.com.
  • Supported by:

    This work was supported by a grant from the Mandatory Project of Health Department of Hebei Province of China, No. 20130027; a grant from the Mandatory Project of Science and Technology Department of Hebei Province of China, No. 142777105D; a grant from Science and Technology Bureau of Chengde City of Hebei Province of China, No. 20123128.

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摘要：

 

实验建立坐骨神经横断大鼠模型，然后随机给予其腹腔注射有神经保护作用的免疫抑制剂他克莫司和/或造成脑挫裂伤。术后4，8和12周，应用Masson三色染色，苏木素-伊红染色和甲苯胺蓝染色观察到，他克莫司联合脑损伤可减轻损伤侧腓肠肌萎缩和损伤坐骨神经纤维病理损害；还可改善坐骨神经功能和提高损伤侧腓肠肌肌肉湿重。术后8和12周，实验还观察到他克莫司联合脑损伤对损伤坐骨神经的动作电位幅值有提高作用，并使辣根过氧化物酶阳性神经元数量明显增加。他克莫司联合脑损伤对损伤周围神经的修复作用明显优于两者单独作用。实验提示，他克莫司联合脑损伤有助于损伤周围神经的修复。

ORCID:0000-0002-0597-546X(Pei Wang)

关键词: 神经再生, 脑损伤, 周围神经, 他克莫司, 逆行示踪, 肌萎缩, 神经修复

Abstract:

Both brain injury and tacrolimus have been reported to promote the regeneration of injured peripheral nerves. In this study, before transection of rat sciatic nerve, moderate brain contusion was (or was not) induced. After sciatic nerve injury, tacrolimus, an immunosuppressant, was (or was not) intraperitoneally administered. At 4, 8 and 12 weeks after surgery, Masson’s trichrome, hematoxylin-eosin, and toluidine blue staining results revealed that brain injury or tacrolimus alone or their combination alleviated gastrocnemius muscle atrophy and sciatic nerve fiber impairment on the experimental side, simultaneously improved sciatic nerve function, and increased gastrocnemius muscle wet weight on the experimental side. At 8 and 12 weeks after surgery, brain injury induction and/or tacrolimus treatment increased action potential amplitude in the sciatic nerve trunk. Horseradish peroxidase retrograde tracing revealed that the number of horseradish peroxidase-positive neurons in the anterior horn of the spinal cord was greatly increased. Brain injury in combination with tacrolimus exhibited better effects on repair of injured peripheral nerves than brain injury or tacrolimus alone. This result suggests that brain injury in combination with tacrolimus promotes repair of peripheral nerve injury.

Key words: nerve regeneration, brain injury, peripheral nerve, tacrolimus, toluidine blue staining, retrograde tracing, muscle atrophy, neural regeneration