中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2026, Vol. 21 ›› Issue (7): 3122-3129.doi: 10.4103/NRR.NRR-D-24-01445

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

负载山楂叶总黄酮与神经生长因子明胶-京尼平水凝胶有效促进脊髓损伤修复

  

  • 出版日期:2026-07-15 发布日期:2026-04-01
  • 基金资助:
    广西科技基地和人才专项项目（GuiKeAD24010037）和广西卫生健康委自筹经费科研项目（Z - A20241029）

Total flavonoids and nerve growth factor loaded gelatin-ginipin hydrogel for NIR enhanced spinal cord injury repair via inhibiting NF-κB pathway

Yu Liang1, 2, #, Deshuang Xi1, #, Yilin Teng1, Pan Liu3, Yanbing Feng1, 4, Qiumei Huang5, Ming Gao6, *, Shaohui Zong1, 7, *   

  1. 1Department of Spine Osteopathia, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region, China; 
    2Department of Spine Surgery, The Third Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region, China; 
    3Department of Orthopedics, The Third Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan Province, China; 
    4Department of Spine Surgery, The Second Hospital of Shandong University, Jinan, Shandong Province, China; 
    5Department of Anesthesiology, The Third Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region, China; 
    6Life Sciences Institute, Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region, China; 
    7Department of Orthopedics, Wuming Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region, China
  • Online:2026-07-15 Published:2026-04-01
  • Contact: Ming Gao, PhD, gaoming1983125@hotmail.com; Shaohui Zong, MD, xiaohui3008@126.com.
  • Supported by:
    The study was supported by Guangxi Science and Technology Base and Talent Special Project, No. GuiKeAD24010037 (to SZ) and Guangxi Health Commission Self-Funded Research Project, No. Z-A20241029 (to YL).

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摘要：

神经生长因子和山楂叶总黄酮有助于脊髓损伤后神经功能的恢复。然而如何有效地将神经生长因子和山楂叶总黄酮输送到脊髓损伤部位，并确保持续释放减少进一步的损伤，仍具有挑战性。此次实验开发了一种基于明胶-京尼平的水凝胶载体，用于缓释山楂叶总黄酮和神经生长因子。结果显示，这种水凝胶具有良好的可注射性、光热效应和药物缓释性能。这种水凝胶经近红外照射后注射至大鼠脊髓损伤部位，可通过抑制NF-κB信号通路减轻氧化应激和炎症反应，促进血管新生和轴突再生，从而显著改善脊髓损伤大鼠的运动功能恢复。上述结果表明，这种新型水凝胶能够改善脊髓损伤的预后，这位脊髓损伤的治疗提供了一种新策略。


https://orcid.org/0000-0002-7454-4815 (Ming Gao); https://orcid.org/0009-0004-3634-243X (Shaohui Zong)

关键词: 复合水凝胶, 山楂叶总黄酮, NF-κB通路, 光热效应, 活性氧, 脊髓损伤

Abstract: Rat nerve growth factor and total flavonoids from hawthorn leaf contribute to the recovery of neurological function after spinal cord injury, including traumatic, non-traumatic spinal cord injuries. However, it remains challenging to efficiently deliver nerve growth factor and total flavonoids from hawthorn leaf to spinal cord injury sites, ensure their sustained release, and minimize further damage. In the present study, we chose a biocompatible and biodegradable gelatin as the substrate, which was crosslinked with the natural biological crosslinker genipin to form a gelatin–genipin hydrogel carrier for the slow release of nerve growth factor and total flavonoids from hawthorn leaf in spinal cord injury sites. The prepared gelatin–genipin hydrogel had good injectable properties and photothermal effects. Furthermore, when the hydrogel with 2% genipin, 200 ng/mL nerve growth factor, and 320 μg/mL total flavonoids from hawthorn leaf was combined with near infrared irradiation, there was a slow release of total flavonoids from hawthorn leaf and nerve growth factor, reduced oxidative stress, an improved inflammatory microenvironment, and accelerated angiogenesis and axonal regeneration via inhibition of the nuclear factor kappa-B signaling pathway, thereby promoting recovery from spinal cord injury. Collectively, our results indicate that this new hydrogel may improve the prognosis of spinal cord injury, and may represent a new strategy for treating spinal cord injury.

Key words: composited hydrogel, hawthorn leaf total flavonoids, nuclear factor kappa-B pathway, photothermal effect, reactive oxygen species, spinal cord injury