中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2018, Vol. 13 ›› Issue (8): 1384-1389.doi: 10.4103/1673-5374.235251

• 原著:退行性病与再生 • 上一篇    下一篇

重复经颅磁刺激对血管性痴呆海马组织胆碱能活性和认知功能的影响

  

  • 收稿日期:2018-03-05 出版日期:2018-08-15 发布日期:2018-08-15
  • 基金资助:

    湖北省教委重大项目（D20152101）

Effects of repetitive transcranial magnetic stimulation on cognitive function and cholinergic activity in the rat hippocampus after vascular dementia

Xiao-Qiao Zhang1, Li Li2, Jiang-Tao Huo1, Min Cheng1, Lin-Hong Li1   

  1. 1 Department of General Medicine & Neurology, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei Province, China;
    2 Department of Pharmacy, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei Province, China
  • Received:2018-03-05 Online:2018-08-15 Published:2018-08-15
  • Contact: Xiao-Qiao Zhang, Ph.D.,little_bridge@126.com.
  • Supported by:

    This study was supported by a grant from the Major Project of Educational Commission of Hubei Province of China, No. D20152101

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摘要：

重复经颅磁刺激作为一种无创性治疗手段，可以促进脑卒中后的神经功能恢复，但其是否也能促进血管性痴呆后海马组织和认知功能的恢复，目前尚不清楚。实验采用两血管闭塞方法建立血管性痴呆模型大鼠，损伤后第2天开始给予重复经颅磁刺激治疗，刺激频率为0.5Hz，磁场强度为1.33 T，2次/d，双侧大脑半球各一次，每次30个脉冲。应用Morris水迷宫实验评估学习记忆功能；以分光光度法测量大脑海马组织乙酰胆碱酯酶和胆碱乙酰转移酶活性；以Karnovsky-Roots法检测海马CA1区胆碱能神经纤维密度，以免疫组化染色检测海马CA1区中脑源性神经营养因子阳性细胞数量。重复经颅磁刺激治疗30d后，见大鼠学习记忆功能明显改善，海马组织中乙酰胆碱酯酶和胆碱乙酰转移酶活性升高，海马CA1区胆碱能神经纤维密度和脑源性神经营养因子阳性细胞数量增加。表明重复经颅磁刺激可以改善血管性痴呆大鼠的学习记忆能力，其机制可能与其促进海马CA1区脑源性神经营养因子表达恢复的海马胆碱能系统活性有关。

orcid:0000-0001-9579-3362(Xiao-Qiao Zhang)

关键词: 胆碱能系统, 神经营养因子, 海马CA1区, 学习记忆功能, 经颅磁刺激, 痴呆, 神经再生

Abstract:

Repetitive transcranial magnetic stimulation (rTMS) is a non-invasive treatment that can enhance the recovery of neurological function after stroke. Whether it can similarly promote the recovery of cognitive function after vascular dementia remains unknown. In this study,a rat model for vascular dementia was established by the two-vessel occlusion method. Two days after injury, 30 pulses of rTMS were administered to each cerebral hemisphere at a frequency of 0.5 Hz and a magnetic field intensity of 1.33 T. The Morris water maze test was used to evaluate learning and memory function. The Karnovsky-Roots method was performed to determine the density of cholinergic neurons in the hippocampal CA1 region. Immunohistochemical staining was used to determine the number of brain-derived neurotrophic factor (BDNF)-immunoreactive cells in the hippocampal CA1 region. rTMS treatment for 30 days significantly improved learning and memory function, increased acetylcholinesterase and choline acetyltransferase activity, increased the density of cholinergic neurons, and increased the number of BDNF-immunoreactive cells. These results indicate that rTMS can ameliorate learning and memory deficiencies in rats with vascular dementia. The mechanism through which this occurs might be related to the promotion of BDNF expression and subsequent restoration of cholinergic system activity in hippocampal CA1 region.

Key words: nerve regeneration, cholinergic system, neurotrophic factor, hippocampal CA1 region, learning and memory function, repetitive transcranial magnetic stimulation, vascular dementia, neural regeneration