中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2018, Vol. 13 ›› Issue (12): 2200-2208.doi: 10.4103/1673-5374.241472

• 原著:脊髓损伤修复保护与再生 • 上一篇    

黄芩的黄酮提取物治疗大鼠脊髓损伤

  

  • 收稿日期:2018-08-20 出版日期:2018-12-15 发布日期:2018-12-15
  • 基金资助:

    中国国家自然科学基金项目（81403278）；陕西省自然科学基金项目（2017JM8058）；陕西省中医药管理局项目（15-ZY038）

Huangqin flavonoid extraction for spinal cord injury in a rat model

Qian Zhang1, Li-Xin Zhang2, Jing An1, Liang Yan3, Cui-Cui Liu1, Jing-Jing Zhao1, Hao Yang1   

  1. 1 Translational Medicine Center, Hong Hui Hospital, Xi’an Jiaotong University, Xi’an, Shaanxi Province, China
    2 Department of Clinical Pharmacy, Hong Hui Hospital, Xi’an Jiaotong University, Xi’an, Shaanxi Province, China
    3 Department of Spine Surgery, Hong Hui Hospital, Xi’an Jiaotong University, Xi’an, Shaanxi Province, China
  • Received:2018-08-20 Online:2018-12-15 Published:2018-12-15
  • Contact: Qian Zhang, PhD, zq-melody@163.com; Hao Yang, PhD, yanghao.71_99@yahoo.com.
  • Supported by:

    This study was supported by the National Natural Science Foundation of China, No. 81403278 (to QZ); the Natural Science Foundation of Shaanxi Province of China, No. 2017JM8058 (to QZ); the Shaanxi Administration of Traditional Chinese Medicine in China, No. 15-ZY038 (to QZ).

PDF

331

摘要：

从中药黄芩中提取的黄酮类化合物具有抗炎作用，被认为可用于治疗脊髓损伤。为验证这一假设，实验以Allen重物坠落法损伤大鼠T9-10段脊髓，建立脊髓挫伤模型，造模前连续1周灌胃12.5 g/kg黄酮提取物，同时在造模后继续灌胃28d，并以造模后30min尾静脉注射甲基强的松龙30 mg/kg作为阳性对照。（1）以BBB评分评价大鼠后肢运动功能，以苏木精-伊红染色检测损伤脊髓组织的病理变化，以免疫荧光染色和Western blot检测损伤脊髓组织中脑源性神经营养因子、神经元标志物神经丝蛋白、小胶质细胞标记物CD11b以及星形胶质细胞标志物胶质纤维酸性蛋白的免疫阳性反应和表达水平；（2）发现经黄芩黄酮提取物治疗的大鼠，脊髓血肿明显减轻，炎性细胞浸润、空腔及瘢痕减少，BBB评分明显增加，且作用与甲基强的松龙接近；（3）经黄芩黄酮提取物治疗的大鼠损伤脊髓组织中脑源性神经营养因子和神经丝蛋白免疫阳性反应和表达水平增加，而CD11b和胶质纤维酸性蛋白免疫阳性反应和表达水平降低，说明黄芩的黄酮提取物可通过诱导脑源性神经营养因子和神经丝蛋白表达，减少小胶质细胞激活以及对反应性星形胶质细胞发挥调节作用来促进脊髓损伤的恢复。

orcid:0000-0002-3859-6503(Qian Zhang)
        0000-0002-1144-0584(Hao Yang)

关键词: 脊髓损伤, 黄酮, 黄芩, 神经丝蛋白, 脑源性神经营养因子, 星形胶质细胞, 小胶质细胞, 神经再生

Abstract:

Flavonoids from Huangqin (dried roots of Scutellaria baicalensis Georgi) have anti-inflammatory effects, and are considered useful for treatment of spinal cord injury. To verify this hypothesis, the T9–10 spinal cord segments of rats were damaged using Allen’s method to establish a rat spinal cord injury model. Before model establishment, Huangqin flavonoid extraction (12.5 g/kg) was administered intragastrically for 1 week until 28 days after model establishment. Methylprednisolone (30 mg/kg) was injected into the tail vein at 30 minutes after model establishment as a positive control. Basso, Beattie, and Bresnahan locomotor scale scores were used to assess hind limb motor function. Hematoxylin-eosin staining was used to detect pathological changes in the injured spinal cord. Immunofluorescence and western blot assays were performed to measure immunoreactivity and expression levels of brain-derived neurotrophic factor, neuronal marker neurofilament protein, microglial marker CD11b and astrocyte marker glial fibrillary acidic protein in the injured spinal cord. Huangqin flavonoid extraction markedly reduced spinal cord hematoma, inflammatory cell infiltration and cavities and scars, and increased the Basso, Beattie, and Bresnahan locomotor scale scores; these effects were identical to those of methylprednisolone. Huangqin flavonoid extraction also increased immunoreactivity and expression levels of brain-derived neurotrophic factor and neurofilament protein, and reduced immunoreactivity and expression levels of CD11b and glial fibrillary acidic protein, in the injured spinal cord. Overall, these data suggest that Huangqin flavonoid extraction can promote recovery of spinal cord injury by inducing brain-derived neurotrophic factor and neurofilament protein expression, reducing microglia activation and regulating reactive astrocytes.

Key words: nerve regeneration, spinal cord injury, flavonoids, Scutellaria baicalensis, neurofilament, brain-derived neurotrophic factor, astrocytes, microglia, neural regeneration