中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2020, Vol. 15 ›› Issue (3): 482-490.doi: 10.4103/1673-5374.266064

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

白藜芦醇恢复脊髓损伤大鼠神经功能恢复及提高抗氧化能力的Meta分析

  

  • 出版日期:2020-03-15 发布日期:2020-05-26
  • 基金资助:
    国家自然科学基金（81873317，81704096，81603635）; 中国上海市中医药重点项目市科学技术委员会基金（16401970100）; 中国慢性病上海中医医疗中心基金（2017ZZ01010）; 国家第十三届中国新药创新与发展重大专项项目（2017ZX09304001）

Neurological recovery and antioxidant effects of resveratrol in rats with spinal cord injury: a meta-analysis

Bao-Ping Xu1, 2, Min Yao1, 3, Zhen-Jun Li1, 4, Zi-Rui Tian1, 3, Jie Ye5, Yong-Jun Wang1, 3, Xue-Jun Cui1, 3   

  1. 1 Spine Disease Institute, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China
    2 Lu’an Hospital of Traditional Chinese Medicine, Lu’an, Anhui Province, China
    3 Key Laboratory of Theory and Therapy of Muscles and Bones, Ministry of Education (Shanghai University of Traditional Chinese Medicine), Shanghai, China
    4 Gansu Provincial Hospital of Traditional Chinese Medicine, Lanzhou, Gansu Province, China
    5 Department of Orthopedics and Traumatology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China
  • Online:2020-03-15 Published:2020-05-26
  • Contact: Xue-Jun Cui, PhD,13917715524@139.com.
  • Supported by:
    This work was supported by the National Natural Science Foundation of China, No. 81873317 (to XJC), No. 81704096 (to MY), No. 81603635 (to JY); a grant from the Municipal Science and Technology Commission of Shanghai-TCM Key Project in China, No. 16401970100 (to YJW); a grant from the Shanghai TCM Medical Center of Chronic Disease in China, No. 2017ZZ01010 (to YJW); the National Thirteenth Five-Year Science and Technology Major Special Project for New Drug Innovation and Development in China, No. 2017ZX09304001 (to YJW).

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摘要：

目的：系统评估白藜芦醇对大鼠脊髓损伤的神经功能恢复及抗氧化能力的影响。

资料来源：以“脊髓损伤”、“白藜芦醇”和“动物实验”为主要检索词，计算机检索PubMed, Embase, EBSCO, Web of Science, Science Direct, CNKI, Wan Fang, VIP和SinoMed databases数据库，搜集有关白藜芦醇治疗大鼠脊髓损伤的动物实验研究，检索时限均从建库至2017年4月，文献语种没有限制。

资料选择：采用“2009年卒中治疗专业学术圆桌会议临床前推荐意见（Stroke Therapy Academic Industry Roundtable recommendations, STAIR）”对文献的质量进行评估。由2名研究者根据文题、摘要和全文独立筛选文献，提取相关数据并评价纳入研究的偏倚风险。采用RevMan 5.3软件对结局指标进行Meta分析。

结局评价指标：采用BBB评分、斜坡实验和Gale运动功能评分评估神经功能。分子生物学检测脊髓组织中氧化应激生物标记物-超氧化物歧化酶、丙二醛、一氧化氮合酶、一氧化氮、黄嘌呤氧化酶和谷胱甘肽水平。

结果：文献质量评估结果提示纳入的12研究文献质量较差。Meta分析结果显示，与对照组相比，白藜芦醇显著提高脊髓损伤后BBB评分[n=300, MD=3.85，95% CI（2.10, 5.59）,P =0.0001]，差异有统计学意义。与对照组相比，白藜芦醇治疗组脊髓组织超氧化物歧化酶水平显著提高[n=138,SMD=5.22，95% CI（2.98，7.45）,P <0.00001]，脊髓组织丙二醛水平显著降低[n=84,SMD=-3.64，95% CI（-5.84，-1.43）,P =0.001]。

结论：白藜芦醇能够促进大鼠脊髓损伤后神经功能恢复，发挥抗氧化作用，受纳入研究的质量所限，需要更多高质量研究为脊髓损伤临床前治疗提供更严谨客观的证据。

orcid: 0000-0002-9006-4547 (Xue-Jun Cui) 

         0000-0001-6257-2766 (Bao-Ping Xu)

关键词: 神经再生, Meta分析, 白藜芦醇, 脊髓损伤, 系统回顾, 神经恢复, 抗氧化, 大鼠, 神经再生, 药物治疗

Abstract:

Objective: To critically assess the neurological recovery and antioxidant effects of resveratrol in rat models of spinal cord injury.

Data sources: Using “spinal cord injury”, “resveratrol” and “animal experiment” as the main search terms, all studies on the treatment of spinal cord injury in rats by resveratrol were searched for in PubMed, EMBASE, MEDLINE, Web of Science, Science Direct, China National Knowledge Infrastructure, Wanfang, VIP, and SinoMed databases by computer. The search was conducted from their inception date to April 2017. No language restriction was used in the literature search. 

Data selection: The methodological quality of each study was assessed by the initial Stroke Therapy Academic Industry Roundtable recommendations. Two reviewers independently selected studies according to the title, abstract and full text. The risk of bias in the included studies was also evaluated. Meta-analyses were performed with Review Manager 5.3 software. 

Outcome measures: Neurological function was assessed by the Basso, Beattie, and Bresnahan scale score, inclined plane score and Gale’s motor function score. Molecular-biological analysis of antioxidative effects was conducted to determine superoxide dismutase levels, malondialdehyde levels, nitric oxide synthase activity, nitric oxide levels, xanthine oxidase and glutathione levels in spinal cord tissues. 

Results: The methodological quality of the 12 included studies was poor. The results of meta-analysis showed that compared with the control group, resveratrol significantly increased the Basso, Beattie, and Bresnahan scale scores after spinal cord injury (n = 300, mean difference (MD) = 3.85, 95% confidence interval (CI) [2.10, 5.59], P < 0.0001). Compared with the control group, superoxide dismutase levels were significantly elevated (n = 138, standardized mean difference (SMD) = 5.22, 95% CI [2.98, 7.45], P < 0.00001), but malondialdehyde levels were significantly diminished (n = 84, SMD = –3.64, 95% CI [–5.84, –1.43], P = 0.001) in the spinal cord of the resveratrol treatment group. 

Conclusions: Resveratrol promoted neurological recovery and exerted antioxidative effects in rat models of spinal cord injury. The limited quality of the included studies reduces the application of this meta-analysis. Therefore, more high-quality studies are needed to provide more rigorous and objective evidence for the pre-clinical treatment of spinal cord injury.

Key words: antioxidation, meta-analysis, neurological recovery, pharmacotherapy, rats, resveratrol, spinal cord injury, systematic review