中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2022, Vol. 17 ›› Issue (6): 1310-1317.doi: 10.4103/1673-5374.327355

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

对侧初级感觉皮质参与电针治疗介导局灶性单侧初级运动皮质梗死后的恢复

  

  • 出版日期:2022-06-15 发布日期:2021-12-17

Contralateral S1 function is involved in electroacupuncture treatment-mediated recovery after focal unilateral M1 infarction

Lu-Lu Yao1, Si Yuan1, Zhen-Nan Wu1, Jian-Yu Luo1, Xiao-Rong Tang1, Chun-Zhi Tang1, Shuai Cui1, 2, *, Neng-Gui Xu1, *   

  1. 1South China Research Center for Acupuncture and Moxibustion, Medical College of Acupuncture Moxibustion and Rehabilitation, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong Province, China; 2Research Institute of Acupuncture and Meridian, Anhui University of Chinese Medicine, Hefei, Anhui Province, China
  • Online:2022-06-15 Published:2021-12-17
  • Contact: Neng-Gui Xu, PhD, ngxu8018@gzucm.edu.cn; Shuai Cui, PhD, cuishuai@gzucm.edu.cn.
  • Supported by:
    This work was supported by Guangzhou University of Chinese Medicine and by grants from General Program of the National Natural Science Foundation of China (No. 81774406, to NGX), Youth Program of the National Natural Science Foundation of China (No. 82004469, to LLY), Fellowship of China postdoctoral Science Foundation (No. 2020M672601, to LLY), Opening Operation Program of Key Laboratory of Acupuncture and Moxibustion of Traditional Chinese Medicine in Guangdong (No. 2017B030314143, to NGX). 

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摘要：

针刺百会（GV20）和大椎（GV14）穴位已被证明可以促进脑卒中后的功能恢复，然而对侧初级感觉皮质是否对这种恢复仍在进一步研究中。实验采用光栓法诱导单侧初级运动皮质局部缺血性梗死小鼠模型后，对其进行电针百会（GV20）和大椎（GV14）穴干预，用体内和体外电生理记录技术检测对侧初级感觉皮质和初级运动皮质神经元的活动和功能。结果显示，单侧初级运动皮质梗死后，对侧初级感觉皮质和初级运动皮质的血液灌注明显减少，内在的神经元兴奋性和活动出现紊乱，而电针治疗可挽救这种紊乱。此外，以病毒感染技术对对侧初级感觉皮质神经元进行消融可抑制电针的治疗作用。结果表明，对侧初级感觉皮质的神经元活动对电针治疗介导的初级运动皮质局灶性梗死的恢复很重要，初级感觉皮质-初级运动皮质回路可能参与电针治疗单侧脑梗塞的作用机制。

https://orcid.org//0000-0002-7945-9177 (Neng-Gui Xu); http://orcid.org/0000-0003-1457-6759 (Shuai Cui)

Abstract: Acupuncture at acupoints Baihui (GV20) and Dazhui (GV14) has been shown to promote functional recovery after stroke. However, the contribution of the contralateral primary sensory cortex (S1) to recovery remains unclear. In this study, unilateral local ischemic infarction of the primary motor cortex (M1) was induced by photothrombosis in a mouse model. Electroacupuncture (EA) was subsequently performed at acupoints GV20 and GV14 and neuronal activity and functional connectivity of contralateral S1 and M1 were detected using in vivo and in vitro electrophysiological recording techniques. Our results showed that blood perfusion and neuronal interaction between contralateral M1 and S1 is impaired after unilateral M1 infarction. Intrinsic neuronal excitability and activity were also disturbed, which was rescued by EA. Furthermore, the effectiveness of EA treatment was inhibited after virus-mediated neuronal ablation of the contralateral S1. We conclude that neuronal activity of the contralateral S1 is important for EA-mediated recovery after focal M1 infarction. Our study provides insight into how the S1–M1 circuit might be involved in the mechanism of EA treatment of unilateral cerebral infarction. The animal experiments were approved by the Committee for Care and Use of Research Animals of Guangzhou University of Chinese Medicine (approval No. 20200407009) April 7, 2020.

Key words:  , brain plasticity, electroacupuncture, electrophysiology recording, neuronal activity, primary motor cortex, primary sensory cortex, stroke 

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