中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (22): 2060-2068.doi: 10.3969/j.issn.1673-5374.2013.22.005

• 原著:神经损伤修复保护与再生 • 上一篇    下一篇

中药复方舒郁散含药血清高浓度皮质酮损伤的SH-SY5Y细胞

  

  • 收稿日期:2012-10-15 修回日期:2013-03-25 出版日期:2013-08-05 发布日期:2013-08-05

Shuyusan-containing serum protects SH-SY5Y cells against corticosterone-induced impairment

Liping Chen1, Zhigao Sun2, Fawei Wang1, Chengyong Xu2, Miao Geng3, Hongyan Chen3, Dongmei Duan1   

  1. 1 Department of Traditional Chinese Medicine, Hainan Branch of Chinese PLA General Hospital, Sanya 572004, Hainan Province, China
    2 Department of Internal Medicine, Hainan Branch of Chinese PLA General Hospital, Sanya 572004, Hainan Province, China
    3 Institute of Geriatrics, General Hospital of Chinese PLA, Beijing 100853, China
  • Received:2012-10-15 Revised:2013-03-25 Online:2013-08-05 Published:2013-08-05
  • Contact: Liping Chen, M.D., Chief physician, Master’s supervisor, Department of Traditional Chinese Medicine, Hainan Branch of Chinese PLA General Hospital, Sanya 572004, Hainan Province, China, lipingschen@yahoo.com.cn.

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摘要：

以往研究显示，以主要有效成分栀子苷为主要的中药复方舒郁散对皮质酮诱导的SH-SY5Y细胞损伤具有保护作用，实验以5，30μg/mL舒郁散含药血清预处理400μM浓度皮质酮诱导损伤的SH-SY5Y细胞，结果显示舒郁散含药血清可提高皮质酮损伤SH-SY5Y细胞的存活率，减少Bax表达，增加Bcl-2表达；可显著提高脑源性神经营养因子的mRNA表达水平，阻滞细胞凋亡，高剂量（30μg/mL）舒郁散含药血清作用更明显。由此认为舒郁散含药血清可能通过影响高浓度皮质酮损伤的SH-SY5Y细胞中凋亡相关蛋白和脑源性神经营养因子的表达，对损伤产生保护作用，且高浓度舒郁散含药血清对高浓度皮质酮损伤的干预效果更为明显。

关键词: 神经再生, 中医药, 舒郁散, 含药血清, SH-SY5Y, 皮质酮, Bcl-2, Bax, 蛋白表达, 凋亡, 脑源性神经营养因子, 基金资助文章

Abstract:

The Chinese herb Shuyusan, whose main constituent is jasminoidin, has been shown to protect SH-SY5Y cells against corticosterone-induced damage. SH-SY5Y cells injured by 400 μmol/L corticosterone were treated with 5 and 30 μg/mL Shuyusan-containing serum. Results revealed that Shuyusan-containing serum elevated the survival rate of SH-SY5Y cells, reduced Bax expression, increased Bcl-2 expression, markedly elevated brain-derived neurotrophic factor mRNA expression, and blocked cell apoptosis. Moreover, the effect of high-dose (30 μg/mL) Shuyusan-containing se-rum was more remarkable. Therefore, Shuyusan-containing serum appears to protect SH-SY5Y cells against corticosterone-induced impairment by adjusting the expression of apoptosis-associ- ated proteins and brain-derived neurotrophic factor. Moreover, high-dose Shuyusan-containing se-rum has a protective effect on high-dose corticosterone-induced impairment.