中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (27): 2510-2519.doi: 10.3969/j.issn.1673-5374.2013.27.002

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

熊果酸诱导损伤坐骨神经的再生

  

  • 收稿日期:2013-03-25 修回日期:2013-07-01 出版日期:2013-09-25 发布日期:2013-09-25
  • 基金资助:

    吉林省科技厅项目资助（No.200705318）

Ursolic acid induces neural regeneration after sciatic nerve injury

Biao Liu1, Yan Liu1, Guang Yang1, Zemin Xu1, Jiajun Chen2   

  1. 1 Department of Hand Surgery, China-Japan Union Hospital of Jilin University, Changchun 130033, Jilin Province, China

    2 Department of Neurology, China-Japan Union Hospital of Jilin University, Changchun 130033, Jilin Province, China
  • Received:2013-03-25 Revised:2013-07-01 Online:2013-09-25 Published:2013-09-25
  • Contact: Jiajun Chen, M.D., Professor, Chief physician, Department of Neurology, China-Japan Union Hospital of Jilin University, Changchun 130033, Jilin Province, China, chenjiajun999@sina.com.
  • About author:Biao Liu, Associate chief physician, Associate professor.
  • Supported by:

    This study was financially sponsored by the Science and Technology Ministry of Jilin Province, No. 200705318.

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摘要：

实验观察具有促进损伤神经元修复的熊果酸对损伤坐骨神经再生的影响。于BALB/c小鼠坐骨结节下0.5cm水平行单侧坐骨神经完全离断后显微吻合制备坐骨神经损伤模型，造模成功后，每天腹腔注射10，5，2.5 mg/kg熊果酸进行干预。ELISA结果显示，坐骨神经损伤后1~4周血清S100蛋白含量逐渐增高，至损伤后8周明显下降；同时熊果酸可显著提高坐骨神经损伤小鼠血清S100蛋白浓度。实时定量PCR结果显示，熊果酸可促进坐骨神经损伤侧脊髓L4-6节段S100 mRNA的表达。肌肉/体质量指数检测发现，熊果酸可提高小鼠比目鱼肌肌肉/体质量指数。甲苯胺蓝染色显示经熊果酸干预的小鼠损伤坐骨神经有髓神经纤维数目及其平均直径明显增大。以上指标均以10，5 mg/kg熊果酸作用更明显。可见熊果酸有剂量依赖性促进坐骨神经损伤小鼠S100的表达促进髓鞘再生的作用。
 

关键词: 神经再生, 中医药, 熊果酸, 三萜系化合物, 坐骨神经, 周围神经损伤, S100, 肌肉萎缩, 神经髓鞘, 基金资助文章

Abstract:

In this study, we aimed to explore the role of ursolic acid in the neural regeneration of the injured sciatic nerve. BALB/c mice were used to establish models of sciatic nerve injury through unilateral sciatic nerve complete transection and microscopic anastomosis at 0.5 cm below the ischial tuber-osity. The successfully generated model mice were treated with 10, 5, or 2.5 mg/kg ursolic acid via intraperitoneal injection. Enzyme-linked immunosorbent assay results showed that serum S100 protein expression level gradually increased at 1–4 weeks after sciatic nerve injury, and significantly decreased at 8 weeks. As such, ursolic acid has the capacity to significantly increase S100 protein expression levels. Real-time quantitative PCR showed that S100 mRNA expression in the L4–6 segments on the injury side was increased after ursolic acid treatment. In addition, the muscular mass index in the soleus muscle was also increased in mice treated with ursolic acid. Toluidine blue staining revealed that the quantity and average diameter of myelinated nerve fibers in the injured sciatic nerve were significantly increased after treatment with ursolic acid. 10 and 5 mg/kg of ursolic acid produced stronger effects than 2.5 mg/kg of ursolic acid. Our findings indicate that ursolic acid can dose-dependently increase S100 expression and promote neural regeneration in BALB/c mice following sciatic nerve injury.

Key words: neural regeneration, traditional Chinese medicine, ursolic acid, triterpenoid, sciatic nerve, peripheral nerve injury, S100, muscular atrophy, nerve myelin sheath, grants-supported paper, neuroregeneration