中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (2): 164-170.doi: 10.4103/1673-5374.125345

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

百会和大椎穴针刺如何减少海洛因复吸的脑细胞凋亡？

  

  • 收稿日期:2013-12-20 出版日期:2014-01-10 发布日期:2014-01-10

The optimal distance between two electrode tips during recording of compound nerve action potentials in the rat median nerve

Xiaorong Hou1, Rongjun Zhang2, Hang Lv3, Xinghui Cai1, Guangchuan Xie3, Xiaoge Song1   

  1. 1 Research Institute of Acupuncture and Meridian, Anhui University of Traditional Chinese Medicine, Anhui Key Laboratory of Foundation and Technology of Acupuncture and Moxibustion (Cultivating Base), Hefei, Anhui Province, China
    2 Department of Human Anatomy, School of Integrated Traditional Chinese & Western Medicine, Anhui University of Traditional Chinese Medicine, Hefei, Anhui Province, China
    3 Department of Acupuncture and Tuina, School of Acupuncture & Osteology, Anhui University of Traditional Chinese Medicine, Hefei, Anhui Province, China
  • Received:2013-12-20 Online:2014-01-10 Published:2014-01-10
  • Contact: Xiaoge Song, Research Institute of Acupuncture and Meridian, Anhui University of Traditional Chinese Medicine, Anhui Key Laboratory of Foundation and Technology of Acupuncture and Moxibustion (Cultivating Base), Hefei 230038, Anhui Province, China, zsongxg0128@163.com.

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摘要：

研究发现针刺百会、大椎穴，可使脑缺血大鼠神经元的丢失减少，超微结构损伤减轻，但针刺治疗成瘾及抗复吸是否通过改善大鼠脑细胞超微结构的变化而达成呢？实验建立海洛因复吸大鼠模型，透视电镜观察海洛因复吸大鼠大脑海马和额叶出现细胞凋亡，免疫组化染色检测显示大脑海马和额叶中抑凋亡因子Bcl-2表达明显减少，促凋亡因子Bax表达明显增加。针刺百会和大椎后，大脑海马和额叶病理损伤明显减轻，大脑海马和额叶中Bcl-2表达增加，Bax表达减少，且作用效果与临床常用治疗吸毒成瘾的药物美沙酮的效果接近。说明针刺大椎、百会穴具有抗海洛因复吸大鼠脑神经细胞凋亡作用。

关键词: 神经再生, 中医药, 针刺, 海洛因复吸, 脑损伤, 超微结构, Bcl-2, Bax, 细胞凋亡, 神经元保护

Abstract:

Acupuncture at Baihui (GV20) and Dazhui (GV14) reduces neuronal loss and attenuates ultrastructural damage in cerebral ischemic rats. However, whether acupuncture can treat addiction and prevent readdiction through changes to brain cell ultrastructure remains unknown. In this study, cell apoptosis was observed in the hippocampus and frontal lobe of heroin readdicted rats by electron microscopy. Immunohistochemical staining displayed a reduction in Bcl-2 expression and an increase in Bax expression in the hippocampus and frontal lobe. After rats were given acupuncture at Baihui and Dazhui, the pathological damage in the hippocampus and frontal lobe was significantly reduced, Bcl-2 expression was upregulated and Bax expression was downregulated. Acupuncture exerted a similar effect with methadone, a commonly used drug for clinical treatment of drug addiction. Experimental findings suggest that acupuncture at Dazhui and Baihui can prevent brain cell apoptosis in heroin readdicted rats.

Key words: nerve regeneration, traditional Chinese medicine, acupuncture, heroin readdiction, brain injury, ultrastructure, Bcl-2, Bax, apoptosis, neuroprotection, NSFC grant, neural regeneration

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