中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (10): 869-881.doi: 10.3969/j.issn.1673-5374.2013.10.001

• 原著:神经损伤修复保护与再生 •    下一篇

血小板反应蛋白1促进骨髓源性神经元样细胞的突触形成

  

  • 收稿日期:2012-12-01 修回日期:2013-03-07 出版日期:2013-04-05 发布日期:2013-04-05

Thrombospondin 1 promotes synaptic formation in bone marrow-derived neuron-like cells

Yun Huang, Mingnan Lu, Weitao Guo, Rong Zeng, Bin Wang, Huaibo Wang   

  1. Department of Orthopedics, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, Guangdong Province, China
  • Received:2012-12-01 Revised:2013-03-07 Online:2013-04-05 Published:2013-04-05
  • Contact: Weitao Guo, Ph.D., Chief physician, Department of Orthopedics, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, Guangdong Province, China, guoweitao2000@sina.com.
  • About author:Yun Huang★, Studying for master’s degree.
  • Supported by:
    广东省自然科学基金项目(No.S2011010004096)；广东省医学科研基金资助项目(No.A2009477，A2010431)。
     

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摘要：

实验以生长因子为诱导剂，诱导骨髓间充质干细胞向神经元样细胞分化，同时加入血小板反应蛋白1观察其对此神经元样细胞突触形成的作用。结果显示，添加血小板反应蛋白1诱导的骨髓间充质干细胞与未添加血小板反应蛋白1的诱导组比较，神经元样细胞的分化率虽无显著差异，但形态要复杂的多，其突起呈树枝状、单极、双极和多极等，更接近正常神经突起的形态。免疫组织化学染色结果显示，血小板反应蛋白1诱导的骨髓间充质干细胞中与突触功能相关的突触后致密蛋白95、突触素1蛋白表达的阳性细胞率上显著提高。说明血小板反应蛋白1对骨髓间充质干细胞诱导分化的神经元样细胞的突触形成有促进作用。

关键词: 神经再生, 干细胞, 骨髓间充质干细胞, 神经元样细胞, 突触形成, 血小板反应蛋白1, 突起, 突触后致密蛋白95, 突触素1, 基金资助文章

Abstract:

In this study, a combination of growth factors was used to induce bone marrow mesenchymal stem cells differentiation into neuron-like cells, in a broader attempt to observe the role of thrombospondin 1 in synapse formation. Results showed that there was no significant difference in the differentiation rate of neuron-like cells between bone marrow mesenchymal stem cells with thrombospondin induction and those without. However, the cell shape was more complex and the neurites were dendritic, with unipolar, bipolar or multipolar morphologies, after induction with thrombospondin 1. The induced cells were similar in morphology to normal neurites. Immunohistochemical staining showed that the number of positive cells for postsynaptic density protein 95 and synaptophysin 1 protein was significantly increased after induction with thrombospondin 1. These findings indicate that thrombospondin 1 promotes synapse formation in neuron-like cells that are differentiated from bone marrow mesenchymal stem cells.

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