中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (10): 890-899.doi: 10.3969/j.issn.1673-5374.2013.10.003

• 原著:神经损伤修复保护与再生 • 上一篇    下一篇

脐带华通胶来源少突胶质前体细胞移植损伤脊髓后轴突和髓鞘的再生

  

  • 收稿日期:2012-12-10 修回日期:2013-02-15 出版日期:2013-04-05 发布日期:2013-04-05

Human umbilical cord Wharton’s jelly-derived oligodendrocyte precursor-like cells for axon and myelin sheath regeneration

Hong Chen1, Yan Zhang2, Zhijun Yang2, Hongtian Zhang2   

  1. 1 Department of Anesthesiology, Military General Hospital of Beijing PLA, Beijing 100700, China
    2 Affiliated Bayi Brain Hospital, Military General Hospital of Beijing PLA, Beijing 100700, China
  • Received:2012-12-10 Revised:2013-02-15 Online:2013-04-05 Published:2013-04-05
  • Contact: Hongtian Zhang, Ph.D., Attending physician, Affiliated Bayi Brain Hospital, Military General Hospital of Beijing PLA, Beijing 100700, China, zhanghongtian007@126.com. Zhijun Yang, Ph.D., Associate chief physician, Affiliated Bayi Brain Hospital, Military General Hospital of Beijing PLA, Beijing 100700, China, zhijunyangfmmu@ yahoo.com.cn.
  • About author:Hong Chen★, Master, Associate chief physician.

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摘要：

从人脐带华通胶中分离获得脐带间充质干细胞，将其诱导分化为少突胶质前体细胞样细胞，移植到大鼠脊髓挫伤组织。免疫荧光双标染色显示，移植细胞可在损伤脊髓组织存活，并向成熟和未成熟的少突胶质细胞分化。生物素标记葡聚糖示踪结果显示，细胞移植可提高大鼠脊髓损伤中心区和损伤尾侧皮质脊髓束密度。Luxol fast blue染色及甲苯胺蓝染色显示，细胞移植后脊髓损伤中心及距中心1, 2mm的头侧和尾侧髓鞘明显增多，且免疫荧光染色证实，再生的髓鞘为髓鞘蛋白前脂蛋白阳性的中枢神经系统类型髓鞘。BBB评分显示细胞移植组大鼠神经功能显著改善。说明人脐血间充质干细胞来源的少突胶质前体细胞可促进损伤脊髓轴突和髓鞘的再生。

关键词: 神经再生, 干细胞, 华通胶, 人脐血间充质干细胞, 少突胶质前体细胞样细胞, 脊髓损伤, 轴突, 髓鞘, 种子细胞, 神经修复, 基金资助文章

Abstract:

Human umbilical mesenchymal stem cells from Wharton’s jelly of the umbilical cord were induced to differentiate into oligodendrocyte precursor-like cells in vitro. Oligodendrocyte precursor cells were transplanted into contused rat spinal cords. Immunofluorescence double staining indicated that transplanted cells survived in injured spinal cord, and differentiated into mature and immature oligodendrocyte precursor cells. Biotinylated dextran amine tracing results showed that cell transplantation promoted a higher density of the corticospinal tract in the central and caudal parts of the injured spinal cord. Luxol fast blue and toluidine blue staining showed that the volume of residual myelin was significantly increased at 1 and 2 mm rostral and caudal to the lesion epicenter after cell transplantation. Furthermore, immunofluorescence staining verified that the newly regenerated myelin sheath was derived from the central nervous system. Basso, Beattie and Bresnahan testing showed an evident behavioral recovery. These results suggest that human umbilical mesenchymal stem cell-derived oligodendrocyte precursor cells promote the regeneration of spinal axons and myelin sheaths.