中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (14): 1262-1268.doi: 10.3969/j.issn.1673-5374.2013.14.002

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

纳米微球携神经营养因子3转染许旺细胞修复坐骨神经缺损

  

  • 收稿日期:2012-07-03 修回日期:2013-04-25 出版日期:2013-05-15 发布日期:2013-05-15

Nanoparticles carrying neurotrophin-3-modified Schwann cells promote repair of sciatic nerve defects

Haibin Zong1, Hongxing Zhao2, Yilei Zhao2, Jingling Jia2, Libin Yang2, Chao Ma2, Yang Zhang1, Yuzhen Dong2   

  1. 1 Functional Laboratory, School of Basic Medical Sciences, Xinxiang Medical College, Xinxiang 453003, Henan Province, China
    2 Department of Orthopedics, the First Affiliated Hospital of Xinxiang Medical College, Weihui 453100, Henan Province, China
  • Received:2012-07-03 Revised:2013-04-25 Online:2013-05-15 Published:2013-05-15
  • Contact: Yuzhen Dong, M.D., Associate chief physician, Associate professor, Master’s supervisor, Department of Orthopedics, the First Affiliated Hospital of Xinxiang Medical College, Weihui 453100, Henan Province, China, dongyuzhen1998@163.com.
  • About author:Haibin Zong, Lecturer.
  • Supported by:

    2010年河南省教育厅高校科技创新人才课题(No. 2010HASTIT036)

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摘要：

许旺细胞及其分泌的神经营养因子3参与神经再生过程，但许旺细胞分泌神经营养因子3有时效性；而外源性神经营养因子3在体内又易失活。为此，实验将神经营养因子3基因经纳米微球载体转染入体外培养的许旺细胞，发现神经营养因子3基因成功转入许旺细胞，并持续有效表达。将转染神经营养因子3基因的许旺细胞结合生物可降解聚乳酸-羟基乙酸共聚物管构建移植复合体，修复大鼠10mm缺损坐骨神经，发现移植复合体有助于坐骨神经的电生理肌电、波幅的恢复，促进坐骨神经轴突及髓鞘再生，并减少脊髓运动神经元凋亡。提示移植转染神经营养因子3基因的许旺细胞与桥接复合可以促进损伤神经的再生和修复。

关键词: 神经再生, 周围神经损伤, 神经营养因子3, 纳米微球载体, 许旺细胞, 坐骨神经, 神经电生理, 基因转染, 基金资助文章

Abstract:

Schwann cells and neurotrophin-3 play an important role in neural regeneration, but the secretion of neurotrophin-3 from Schwann cells is limited, and exogenous neurotrophin-3 is inactived easily in vivo. In this study, we have transfected neurotrophin-3 into Schwann cells cultured in vitro using nanoparticle liposomes. Results showed that neurotrophin-3 was successfully transfected into Schwann cells, where it was expressed effectively and steadily. A composite of Schwann cells transfected with neurotrophin-3 and poly(lactic-co-glycolic acid) biodegradable conduits was transplanted into rats to repair 10-mm sciatic nerve defects. Transplantation of the composite scaffold could restore the myoelectricity and wave amplitude of the sciatic nerve by electrophysiological examination, promote nerve axonal and myelin regeneration, and delay apoptosis of spinal motor neurons. Experimental findings indicate that neurotrophin-3 transfected Schwann cells combined with bridge grafting can promote neural regeneration and functional recovery after nerve injury.

Key words: neural regeneration, peripheral nerve injury, neurotrophin-3, nanoparticle liposome, Schwann cells, sciatic nerve, neuroelectrophysiology, gene transfection, grants-supported paper, neuroregeneration