中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (15): 1376-1382.doi: 10.3969/j.issn.1673-5374.2013.15.004

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

柿叶黄酮提高小鼠脑缺血耐受的机制

  

  • 收稿日期:2012-10-25 修回日期:2013-01-08 出版日期:2013-05-25 发布日期:2013-05-25

Persimmon leaf flavonoid induces brain ischemic tolerance in mice

Mingsan Miao, Xuexia Zhang, Linan Wang   

  1. Henan University of Traditional Chinese Medicine, Zhengzhou 450008, Henan Province, China
  • Received:2012-10-25 Revised:2013-01-08 Online:2013-05-25 Published:2013-05-25
  • Contact: Mingsan Miao☆, M.D., Professor, Henan University of Traditional Chinese Medicine, Zhengzhou 450008, Henan Province, China, miaomingsan@163.com

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摘要：

脑缺血耐受机制研究目前较少，有研究显示柿叶有改善脑缺血作用。实验给予脑缺血耐受小鼠模型10 min的缺血预处理，再用柿叶黄酮连续灌胃5 d后发现，柿叶黄酮干预后可显著提高脑缺血耐受小鼠大脑皮质纤溶酶原激活剂和6-酮-前列腺素-F1α含量，降低血栓烷素B2含量，降低血浆纤溶酶原激活剂抑制物1含量。光学显微镜下观察，柿叶黄酮干预可减轻模型小鼠大脑皮质和海马组织中细胞肿胀和核淡染的现象。说明柿叶黄酮可有效抑制脑部血栓的形成，改善脑部血液供应，减轻缺血所致的脑部病理损害，以此提高小鼠脑缺血耐受能力。

关键词: 神经再生, 中医药, 柿叶黄酮, 脑缺血耐受, 缺血预处理, 皮质, 海马, 病理, 纤溶酶原激活剂, 纤溶酶原激活剂抑制物1, 6-酮-前列腺素-F1α, 血栓烷素B2

Abstract:

The persimmon leaf has been shown to improve cerebral ischemic outcomes; however, its mechanism of action remains unclear. In this study, mice were subjected to 10 minutes of ischemic preconditioning, and persimmon leaf flavonoid was orally administered for 5 days. Results showed that the persimmon leaf flavonoid significantly improved the content of tissue type plasminogen activator and 6-keto prostaglandin-F1 α in the cerebral cortex, decreased the content of thromboxane B2, and reduced the content of plasminogen activator inhibitor-1 in mice. Following optical microscopy, persimmon leaf flavonoid was also shown to reduce cell swelling and nuclear hyperchromatism in the cerebral cortex and hippocampus of mice. These results suggested that persimmon leaf flavonoid can effectively inhibit brain thrombosis, improve blood supply to the brain, and relieve ischemia-induced pathological damage, resulting in brain ischemic tolerance.