中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (22): 2086-2092.doi: 10.3969/j.issn.1673-5374.2013.22.008

• 原著:神经损伤修复保护与再生 • 上一篇    下一篇

脐带间充质干细胞诱导的运动神经元样细胞可分泌乙酰胆碱

  

  • 收稿日期:2013-05-08 修回日期:2013-07-25 出版日期:2013-08-05 发布日期:2013-08-05

Acetylcholine secretion by motor neuron-like cells from umbilical cord mesenchymal stem cells

Xueyuan Liu, Dehua Li, Dong Jiang, Yan Fang   

  1. Department of Anatomy, Liaoning Medical University, Jinzhou 121001, Liaoning Province, China
  • Received:2013-05-08 Revised:2013-07-25 Online:2013-08-05 Published:2013-08-05
  • Contact: Dehua Li, Professor, Master’s supervisor, Department of Anatomy, Liaoning Medical University, Jinzhou 121001, Liaoning Province, China, aldh@163.com.
  • About author:Xueyuan Liu, Master, Experimentalist.

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摘要：

实验以双酶消化法从脐带中分离培养脐带间充质干细胞，取第3代细胞加入肝素和/或碱性成纤维细胞生长因子诱导分化，结果发现单独使用碱性成纤维细胞生长因子诱导后细胞形态无明显改变；而使用肝素单独使用或联合碱性成纤维细胞生长因子诱导的细胞呈神经元样形态，神经元特异性标志物微管相关蛋白2表达增加，乙酰胆碱水平随着诱导时间的延长而逐渐增加，且肝素联合碱性成纤维细胞生长因子诱导的细胞高表达运动神经元特异性标志物Hb9及胆碱乙酰转移酶。提示单独使用碱性成纤维细胞生长因子的诱导效果不明显，而肝素联合碱性成纤维细胞生长因子能显著促进脐带间充质干细胞向运动神经元样细胞分化，且能分泌乙酰胆碱。

关键词: 神经再生, 干细胞, 人脐带间充质干细胞, 运动神经元, 肝素, 碱性成纤维细胞生长因子, 诱导, 分化, Hb9, 乙酰胆碱

Abstract:

Umbilical cord mesenchymal stem cells were isolated by a double enzyme digestion method. The third passage of umbilical cord mesenchymal stem cells was induced with heparin and/or basic fi-broblast growth factor. Results confirmed that cell morphology did not change after induction with basic fibroblast growth factor alone. However, neuronal morphology was visible, and microtu-bule-associated protein-2 expression and acetylcholine levels increased following induction with heparin alone or heparin combined with basic fibroblast growth factor. Hb9 and choline acetyltransferase expression was high following inductive with heparin combined with basic fibro-blast growth factor. Results indicate that the inductive effect of basic fibroblast growth factor alone was not obvious. Heparin combined with basic fibroblast growth factor noticeably promoted the dif-ferentiation of umbilical cord mesenchymal stem cells into motor neuron-like cells. Simultaneously, umbilical cord mesenchymal stem cells could secrete acetylcholine.

Key words: neural regeneration, stem cells, human umbilical cord mesenchymal stem cell, motor neuron, heparin, basic fibroblast growth factor, induction, differentiation, Hb9, acetylcholine, neuroregeneration