中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (24): 2236-2248.doi: 10.3969/j.issn.1673-5374.2013.24.003

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

采用星形胶质细胞和核心蛋白聚糖的联合移植修复脊髓挫伤

  

  • 收稿日期:2013-03-15 修回日期:2013-06-27 出版日期:2013-08-25 发布日期:2013-08-25

Combined transplantation of GDAsBMP and hr-decorin in spinal cord contusion repair

Liang Wu1, 2, 3, Jianjun Li1, 2, Liang Chen1, 2, Hong Zhang1, Li Yuan1, 2, Stephen JA Davies4   

  1. 1 School of Rehabilitation Medicine, Capital Medical University, Beijing 100068, China
    2 Department of Neural Functional Reconstruction of Spine and Spinal Cord, China Rehabilitation Research Center, Beijing 100068, China
    3 Rehabilitation Center, Beijing Xiaotangshan Rehabilitation Hospital, Beijing 102211, China
    4 Department of Neurosurgery, University of Colorado Denver, 1250 14th Street Denver, Colorado 80217, USA
  • Received:2013-03-15 Revised:2013-06-27 Online:2013-08-25 Published:2013-08-25
  • Contact: Jianjun Li, Master, Professor, Chief physician, School of Rehabilitation Medicine, Capital Medical University, Beijing 100068, China; Department of Neural Functional Reconstruction of Spine and Spinal Cord, China Rehabilitation Research Center, Beijing 100068, China, lcrrc2007@ yahoo.com.cn; Stephen JA Davies, Ph.D., Associate professor, Department of Neurosurgery, University of Colorado Denver, 1250 14th Street Denver, Colorado 80217, USA, sdavies@bcm.edu.
  • About author:Liang Wu, Ph.D., M.D.
  • Supported by:

    中华人民共和国财政部和中国康复研究中心项目基金资助(2008-2，2008-3，2008-4，2008-5)

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摘要：

脊髓损伤后，星形胶质细胞增殖和瘢痕形成，是抑制脊髓轴突再生和生长的主要因素。而重组人核心蛋白聚糖可以抑制炎症反应及抑制胶质瘢痕的形成，促进轴突生长。鉴于此，实验采用打击法建立T8脊髓挫伤大鼠模型，采用骨形态发生蛋白4诱导并来源于限制性胶质前体的星形胶质细胞和重组人核心蛋白聚糖联合脊髓注射。双重荧光免疫组织化学检测显示，脊髓挫伤28 d后，联合移植法抑制了挫伤大鼠脊髓早期炎症反应，而移植的细胞所分泌的脑源性神经营养因子，保护挫伤脊髓内受伤的轴突。通过抑制挫伤脊髓内星形胶质细胞增殖和胶质瘢痕的形成，促进了损伤脊髓的运动和感觉轴突的再生和生长，并使挫伤脊髓内星形胶质细胞线性排列，为轴突再生提供通道。

关键词: 神经再生, 脊髓损伤, 星形胶质细胞, 胶质瘢痕, 神经干细胞, 联合移植, 神经再生, 神经胶质祖细胞, 神经胶质细胞, 人类重组蛋白聚糖, 脑源性生长因子, 胶质纤维酸性蛋白, 基金资助文章

Abstract:

Following spinal cord injury, astrocyte proliferation and scar formation are the main factors inhibiting the regeneration and growth of spinal cord axons. Recombinant decorin suppresses inflammatory reactions, inhibits glial scar formation, and promotes axonal growth. Rat models of T8 spinal cord contusion were created with the NYU impactor and these models were subjected to combined transplantation of bone morphogenetic protein-4-induced glial-restricted precursor-derived astro-cytes and human recombinant decorin transplantation. At 28 days after spinal cord contusion, dou-ble-immunofluorescent histochemistry revealed that combined transplantation inhibited the early in-flammatory response in injured rats. Furthermore, brain-derived neurotrophic factor, which was se-creted by transplanted cells, protected injured axons. The combined transplantation promoted ax-onal regeneration and growth of injured motor and sensory neurons by inhibiting astrocyte prolifer-ation and glial scar formation, with astrocytes forming a linear arrangement in the contused spinal cord, thus providing axonal regeneration channels.

Key words: neural regeneration, spinal cord injury, astrocytes, glial scar, neural stem cells, combined trans-plantation, glial progenitor cells, glial cells, human recombinant decorin, brain-derived growth factor, glial fibrillary acidic protein, grants-supported paper, neuroregeneration