中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (34): 3255-3262.doi: 10.3969/j.issn.1673-5374.2013.34.010

• 原著:脑损伤修复保护与再生 • 上一篇    

靶向注射无水乙醇建立痉挛型脑瘫大鼠模型

  

  • 收稿日期:2013-08-04 修回日期:2013-11-26 出版日期:2013-12-05 发布日期:2013-12-05

Establishing a rat model of spastic cerebral palsy by targeted ethanol injection

Yadong Yu, Liang Li, Xinzhong Shao, Fangtao Tian, Qinglu Sun   

  1. Department of Hand Surgery, the Third Hospital of Hebei Medical University, Shijiazhuang 050051, Hebei Province, China
  • Received:2013-08-04 Revised:2013-11-26 Online:2013-12-05 Published:2013-12-05
  • Contact: Yadong Yu, M.D., Chief physician, Department of Hand Surgery, the Third Hospital of Hebei Medical University, Shijiazhuang 050051, Hebei Province, China, yuyadong123@ yahoo.cn.

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摘要：

通常认为，痉挛性脑瘫是大脑皮质或锥体束损害的结果。因此，我们利用脑立体定位仪对2月龄SD大鼠左侧锥体束部位进行精确的立体定位，腹腔麻醉后固定于脑立体定位仪上，依大鼠脑立体定位图谱，选择前囟后8.72mm，矢状缝左侧0.8mm颅顶钻孔（直径约1mm），将微量注射器垂直向颅内插入9.7mm缓慢注射15μL无水乙醇已建立痉挛性脑瘫模型。结果显示大鼠大脑锥体束呈空洞样坏死，且大鼠产生典型的屈曲痉挛症状和体征，痉挛持续时间较长。说明这种方法建立的痉挛型脑瘫大鼠模型可复制性良好，且方法简便，无水乙醇作为化学消融剂，对大鼠大脑锥体束造成的破坏彻底而范围局限，对大脑其他部位的影响小，使模型出现典型的屈曲痉挛症状和体征。

中国神经再生研究（英文版）杂志出版内容重点： 脑损伤；脊髓损伤；周围神经损伤；帕金森；神经影像；神经再生

关键词: 神经再生, 脑损伤, 痉挛型脑瘫, 动物模型, 无水乙醇, 锥体束, 脑立体定位仪, 靶向注射, 造模方法

Abstract:

Spastic cerebral palsy is generally considered to result from cerebral cortical or pyramidal tract damage. Here, we precisely targeted the left pyramidal tract of 2-month-old Sprague-Dawley rats placed on a stereotaxic instrument under intraperitoneal anesthesia. Based on the rat brain stere-otaxic map, a 1-mm hole was made 10 mm posterior to bregma and 0.8 mm left of sagittal suture. A microsyringe was inserted perpendicularly to the surface of the brain to a depth of 9.7 mm, and 15 μL of ethanol was slowly injected to establish a rat model of spastic cerebral palsy. After modeling, the rats appeared to have necrotic voids in the pyramidal tract and exhibited typical signs and symptoms of flexion spasms that lasted for a long period of time. These findings indicate that this is an effective and easy method of establishing a rat model of spastic cerebral palsy with good re-producibility. Ethanol as a chemical ablation agent specifically and thoroughly damages the py-ramidal tract, and therefore, the animals display flexion spasms, which are a typical symptom of the disease.

Key words: neural regeneration, brain injury, spastic cerebral palsy, animal models, ethanol, pyramidal tract, stereotaxic instrument, targeted injection, modeling methods, neuroregeneration