中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (35): 3353-3358.doi: 10.3969/j.issn.1673-5374.2013.35.010

• 原著:神经损伤修复保护与再生 • 上一篇    

当归诱导人脂肪源性干细胞向神经元样细胞分化

  

  • 收稿日期:2013-07-16 修回日期:2013-09-20 出版日期:2013-12-15 发布日期:2013-12-15
  • 基金资助:

    四川省科技厅项目 (2009JY0128), 四川省卫生厅项目（20060052）

Differentiation of human adipose-derived stem cells into neuron-like cells by Radix Angelicae Sinensis

Qiaozhi Wang1, Lile Zhou1, Yong Guo1, Guangyi Liu1, Jiyan Cheng2, Hong Yu1   

  1. 1 Department of Histology and Embryology, Luzhou Medical College, Luzhou 646000, Sichuan Province, China
    2 Department of Electronic Microscopy, Luzhou Medical College, Luzhou 646000, Sichuan Province, China
  • Received:2013-07-16 Revised:2013-09-20 Online:2013-12-15 Published:2013-12-15
  • Contact: Hong Yu, Master, Professor, Department of Histology and Embryology, Luzhou Medical College, Luzhou 646000, Sichuan Province, China, yuhong8592@126.com.
  • About author:Qiaozhi Wang, Master, Associate professor.
  • Supported by:

    This study was financially supported by the Science and Technology Project of Sichuan Province, No. 2009JY0128 and the Health Ministry of Sichuan Province in China, No. 20060052.

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摘要：

从成人皮下脂肪组织获得原代人脂肪源性干细胞，采用10%当归注射液预诱导24h后，用含40%当归注射液的无血清DMEM/F12培养基诱导其向神经元样细胞分化，并以常用的神经细胞分化诱导剂丁酸羟基茴香醚作为对照。结果显示，诱导24h后，当归诱导的人脂肪源性干细胞神经元特异性烯醇化酶阳性率不如丁酸羟基茴香醚诱导的高，但细胞存活率明显高于丁酸羟基茴香醚诱导组，且未见神经胶质细胞标志物胶质纤维酸性蛋白的表达；诱导48h，当归诱导的人脂肪源性干细胞神经元特异性烯醇化酶阳性率明显高于丁酸羟基茴香醚诱导组。提示当归可诱导人脂肪源性干细胞向神经元样细胞分化，且细胞毒性小。

中国神经再生研究（英文版）杂志出版内容重点： 脑损伤；脊髓损伤；周围神经损伤；帕金森；神经影像；神经再生

关键词: 神经再生, 干细胞, 中医药, 人脂肪源性干细胞, 当归, 神经元样细胞, 分化, 脂肪细胞, 细胞毒性, 基金资助文章

Abstract:

Human adipose tissues are an ideal source of stem cells. It is important to find inducers that can safely and effectively differentiate stem cells into functional neurons for clinical use. In this study, we investigate the use of Radix Angelicae Sinensis as an inducer of neuronal differentiation. Primary human adipose-derived stem cells were obtained from adult subcutaneous fatty tissue, then pre-induced with 10% Radix Angelicae Sinensis injection for 24 hours, and incubated in serum-free Dulbecco’s modified Eagle’s medium/Nutrient Mixture F-12 containing 40% Radix Angelicae Sinensis to induce its differentiation into neuron-like cells. Butylated hydroxyanisole, a common in-ducer for neuronal differentiation, was used as the control. After human adipose-derived stem cells differentiated into neuron-like cells under the induction of Radix Angelicae Sinensis for 24 hours, the positive expression of neuron-specific enolase was lower than that of the butylated hydroxyanisole-induced group, and the expression of glial fibrillary acidic protein was negative. After they were induced for 48 hours, the positive expression of neuron specific enolase in human adi-pose-derived stem cells was significantly higher than that of the butylated hydroxyanisole-induced group. Our experimental findings indicate that Radix Angelicae Sinensis can induce human adi-pose-derived stem cell differentiation into neuron-like cells and produce less cytotoxicity.

Key words: neural regeneration, stem cells, human adipose-derived stem cells, Radix Angelicae Sinensis, neuron-like cells, differentiation, adipocytes, cytotoxicity, grants-supported paper, neuroregeneration