中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (36): 3382-3390.doi: 10.3969/j.issn.1673-5374.2013.36.003

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

弥散张量成像的数据可反映慢性脊髓损伤的病理变化？

  

  • 收稿日期:2013-08-06 修回日期:2013-11-17 出版日期:2013-12-25 发布日期:2013-12-25

Does diffusion tensor data reflect pathological changes in the spinal cord with chronic injury?

Erjian Lin1, Houqing Long2, Guangsheng Li3, Wanlong Lei4   

  1. 1 Department of Radiology, the Eastern Hospital of the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510700, Guangdong Province, China
    2 Department of Spinal Surgery, Huangpu Branch, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510700, Guangdong Province, China
    3 Department of Orthopedics, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, Guangdong Province, China
    4 Department of Human Anatomy and Histoembryology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510086, Guangdong Province, China
  • Received:2013-08-06 Revised:2013-11-17 Online:2013-12-25 Published:2013-12-25
  • Contact: Houqing Long, M.D., Associate professor, Associate chief physician, Master’s supervisor, Department of Spinal Surgery, Huangpu Branch, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510700, Guangdong Province, China, houqinglong@163.com. Wanlong Lei, M.D., Professor, Doctoral supervisor, Department of Human Anatomy and Histoembryology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510086, Guangdong Province, China, leiwl@mail.sysu.edu.cn.
  • About author:Erjian Lin, Master, Technician-in-charge.

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摘要：

近年有研究指出，磁共振弥散张量成像检测具有对慢性脊髓型颈椎病早期病理改变的进行定量测量的能力。鉴于此，实验将新型吸水聚氨酯材料植入大鼠C3-5硬膜外间隙建立慢性压迫性脊髓损伤大鼠模型，以弥散张量成像数据预测其病理变化。检测结果显示，模型大鼠脊髓各向异性分数值在损伤后4，24，72 h，1周逐渐下降，呈时间依赖性；平均弥散系数值在植入后72 h，1周升高。苏木精-伊红染色及坚牢蓝染色显示，模型大鼠脊髓灰质前角神经元数量及白质神经纤维密度均随脊髓压迫性损伤时间的延长逐渐降低，损伤1周神经元的丢失最为显著。结果证实，弥散张量成像检测中的各向异性分数值及平均弥散系数值能够反映慢性压迫性脊髓损伤大鼠脊髓压迫部位不同时期的病理改变程度，可能对慢性脊髓型颈椎病的早期诊断有参考价值。

中国神经再生研究（英文版）杂志出版内容重点： 脑损伤；脊髓损伤；周围神经损伤；帕金森；神经影像；神经再生

关键词: 神经再生, 神经影像, 磁共振, 压迫性颈脊髓损伤, 病理学, 大鼠, 弥散张量成像, 颈髓, 动物模型, 髓型颈椎病, 神经丝, 基金资助文章

Abstract:

Magnetic resonance diffusion tensor imaging has been shown to quantitatively measure the early pathological changes in chronic cervical spondylotic myelopathy. In this study, a novel spongy pol-yurethane material was implanted in the rat C3–5 epidural space to establish a rat model of chronic cervical spondylotic myelopathy. Diffusion tensor data were used to predict pathological changes. Results revealed that the fractional anisotropy value gradually decreased at 4, 24, and 72 hours and 1 week after injury in rat spinal cord, showing a time-dependent manner. Average diffusion coeffi-cient increased at 72 hours and 1 week after implantation. Hematoxylin-eosin staining and Luxol-fast-blue staining exhibited that the number of neurons in the anterior horn of the spinal cord gray matter and the nerve fiber density of the white matter gradually reduced with prolonged com-pression time. Neuronal loss was most significant at 1 week after injury. Results verified that the fractional anisotropy value and average diffusion coefficient reflected the degree of pathological change in the site of compression in rat models at various time points after chronic spinal cord compression injury, which potentially has a reference value in the early diagnosis of chronic cervical spondylotic myelopathy.