中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (2): 171-178.doi: 10.4103/1673-5374.125346

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

大鼠正中神经复合神经动作电位电极之间最佳距离记录

  

  • 收稿日期:2013-09-01 出版日期:2014-01-10 发布日期:2014-01-10
  • 基金资助:

    复旦大学手功能研究中心基金;国家211工程项目(211XKZ)

The optimal distance between two electrode tips during recording of compound nerve action potentials in the rat median nerve

Yongping Li1, 2, Jie Lao1, Xin Zhao1, Dong Tian1, Yi Zhu1, Xiaochun Wei2   

  1. 1 Department of Hand Surgery of HuaShan Hospital, Fudan University; Key Laboratory of Hand Reconstruction, Ministry of Healthy; Shanghai Key Laboratory of Peripheral Nerve and Microsurgery,  Shanghai, China
    2 Department of Orthopedics, the Second Hospital of Shanxi Medical University, Taiyuan, Shanxi Province, China
  • Received:2013-09-01 Online:2014-01-10 Published:2014-01-10
  • Contact: Jie Lao, Ph.D., Department of Hand Surgery, HuaShan Hospital, No. 12 WuLuMuQi Zhong Road, Shanghai 200040, China, laojie666@163.com.
  • Supported by:

    This study was supported by grants from Hand Function Research Center in Fudan University, China and the Project 211 in China, No. 211XKZ. 

摘要:

一些研究发现记录复合神经动作电位时电极间距离会影响动作电位的幅度。但在较短的神经,小的电极间距离与记录到的复合神经动作电位参数的关系目前尚不清楚。为探索大鼠正中神经在体记录复合神经动作电位的参数与其电极之间距离的关系,我们按不同的正中神经游离方法和电极放置位置,探寻记录复合神经动作电位的最佳方法。同时,行顺向或逆向记录复合神经动作电位,观察他们之间的差异。结果发现,保留正中神经中间系膜,游离神经两端,地线置于神经下系膜组织,能恒定记录到复合神经动作电位。当刺激电极两针之间的距离从1.0mm到5.0mm改变时,记录到的复合神经动作电位的刺激阈值和超强刺激强度逐渐减低,对复合神经动作电位幅度无明显影响;记录到的复合神经动作电位幅度逐渐增加,对复合神经动作电位刺激阈值和超强刺激强度无显著影响。当记录电极和刺激电极之间的距离(8,10,12mm)改变时,记录到的复合神经动作电位各参数无显著变化。由此,我们探寻到了能恒定记录较短神经CNAP的方法。在大鼠正中神经记录复合神经动作电位时,记录电极两个针之间距离5.0mm为最佳,刺激电极两个针之间距离5.0mm为最佳,记录电极和刺激电极之间距离10mm为最适。

关键词: 神经再生, 周围神经损伤, 复合神经动作电位, 正中神经, 电极, 幅度, 超大刺激强度, 记录电极, 国家211工程项目

Abstract:

The distance between the two electrode tips can greatly influence the parameters used for recording compound nerve action potentials. To investigate the optimal parameters for these recordings in the rat median nerve, we dissociated the nerve using different methods and compound nerve action potentials were orthodromically or antidromically recorded with different electrode spacings. Compound nerve action potentials could be consistently recorded using a method in which the middle part of the median nerve was intact, with both ends dissociated from the surrounding fascia and a ground wire inserted into the muscle close to the intact part. When the distance between two stimulating electrode tips was increased, the threshold and supramaximal stimulating intensity of compound nerve action potentials were gradually decreased, but the amplitude was not changed significantly. When the distance between two recording electrode tips was increased, the amplitude was gradually increased, but the threshold and supramaximal stimulating intensity exhibited no significant change. Different distances between recording and stimulating sites did not produce significant effects on the aforementioned parameters. A distance of 5 mm between  recording and stimulating electrodes and a distance of 10 mm between recording and stimulating sites were found to be optimal for compound nerve action potential recording in the rat median nerve. In addition, the orthodromic compound action potential, with a biphasic waveform that was more stable and displayed less interference (however also required a higher threshold and higher supramaximal stimulus), was found to be superior to the antidromic compound action potential.

Key words: nerve regeneration, peripheral nerve injury, compound nerve action potential, median nerve, electrodes, amplitude, supramaximal stimulus intensity, recording electrode, the Project 211 in China, neural regeneration