中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (4): 402-406.doi: 10.4103/1673-5374.128245

• 原著:退行性病与再生 • 上一篇    下一篇

Pael-R基因未参与鱼藤酮诱导帕金森病细胞模型的变化

  

  • 收稿日期:2014-01-19 出版日期:2014-02-25 发布日期:2014-02-25

The Pael-R gene does not mediate the changes in rotenone-induced Parkinson’s disease model cells

Ting Zou, Xiangqi Tang, Zhiling Huang, Niangui Xu, Zhiping Hu   

  1. Department of Neurology, Second Xiangya Hospital, Central South University, Changsha, Hunan Province, China
  • Received:2014-01-19 Online:2014-02-25 Published:2014-02-25
  • Contact: Zhiping Hu, M.D., Department of Neurology, Second Xiangya Hospital, Central South University, Changsha 410011, Hunan Province, China, huzhiping_csu@hotmail.com.

摘要:

实验以鱼藤酮处理后建立帕金森病细胞模型,然后将针对Pael-R基因的RNA干扰载体利用脂质体转染模型细胞。Pael-R基因被干扰后通过RT-PCR和Western bolt法得出与对照组相比Pael-R基因表达量明显减少,但通过流式细胞仪和MTT法未见多巴胺能细胞存活率提高和凋亡率下降。因此认为在鱼藤酮致帕金森病过程中Pael-R基因未参与作用。

关键词: 神经再生, 神经退行性变, 帕金森病, 鱼藤酮, Pael-R, RNA干扰, 细胞凋亡, 线粒体

Abstract:

In this study, we established cell models for Parkinson’s disease using rotenone. An RNA interference vector targeting Parkin-associated endothelin receptor-like receptor (Pael-R) was transfected into the model cells. The results of reverse-transcription polymerase chain reaction and western blot analysis showed that Pael-R expression was decreased after RNA interference compared with the control group (no treatment) and the model group (rotenone treatment), while the rate of apoptosis and survival of dopaminergic cells did not differ significantly between groups, as detected by flow cytometry and an MTT assay. These experimental findings indicate that the Pael-R gene has no role in the changes in rotenone-induced Parkinson’s disease model cells.

Key words: nerve regeneration, neurodegeneration, Parkinson’s disease, rotenone, Pael-R, RNA interference, apoptosis, mitochondria, neural regeneration