中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (7): 719-726.doi: 10.4103/1673-5374.131576

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

正丁基苯酞可使慢性脑缺血后神经元形态趋向正常

  

  • 收稿日期:2014-02-24 出版日期:2014-04-15 发布日期:2014-04-15

3-N-butylphthalide improves neuronal morphology after chronic cerebral ischemia

Wanhong Zhao, Chao Luo, Jue Wang, Jian Gong, Bin Li, Yingxia Gong, Jun Wang, Hanqin Wang   

  1. Institute of Basic Medical Sciences, Hubei University of Medicine, Shiyan, Hubei Province, China
  • Received:2014-02-24 Online:2014-04-15 Published:2014-04-15
  • Contact: Hanqin Wang, M.D., Ph.D., Institute of Basic Medical Sciences, Faculty of Basic Medical Sciences, Hubei University of Medicine, Shiyan 442000, Hubei Province, China, 117298088@qq.com.
  • Supported by:

    This study was financially supported by Innovation Team Project of Hubei Province 2011 Plans, No. 2011JH-2013CXTT06; Momentous Scientific Research Funds of Hubei Provincial Education Ministry, No. D20102101; and Cultivating Funds of Country’s Projects of Hubei University of Medicine, No. 2013GPY03.

摘要:

研究已证实,正丁基苯酞可有效对抗急性缺血性脑卒中,但其对慢性脑缺血导致的神经元损伤的作用尚不知晓。实验结扎老年大鼠双侧颈动脉,以模拟慢性脑缺血老年患者,灌胃给予正丁基苯酞。结果发现,正丁基苯酞干预使慢性脑缺血大鼠大脑皮质和海马神经元形态趋向正常;大脑皮质和海马乙酰胆碱转移酶活性也明显上升;丙二醛和淀粉样β蛋白1-42含量明显降低;认知功能明显改善。这些数据说明正丁基苯酞可减轻慢性脑缺血大脑氧化应激损伤,改善胆碱能神经功能,抑制淀粉样β蛋白积聚,减轻大脑神经元损伤和认知功能损害。

关键词: 神经再生, 正丁基苯酞, 神经元, 认知缺损, 脑缺血, 大脑皮质, 海马, 氧化应激, 胆碱能神经元, 淀粉样β蛋白

Abstract:

3-N-butylphthalide is an effective drug for acute ischemic stroke. However, its effects on chronic cerebral ischemia-induced neuronal injury remain poorly understood. Therefore, this study ligated bilateral carotid arteries in 15-month-old rats to simulate chronic cerebral ischemia in aged humans. Aged rats were then intragastrically administered 3-n-butylphthalide. 3-N-butylphthalide administration improved the neuronal morphology in the cerebral cortex and hippocampus of rats with chronic cerebral ischemia, increased choline acetyltransferase activity, and decreased malondialdehyde and amyloid beta levels, and greatly improved cognitive function. These findings suggest that 3-n-butylphthalide alleviates oxidative stress caused by chronic cerebral ischemia, improves cholinergic function, and inhibits amyloid beta accumulation, thereby improving cerebral neuronal injury and cognitive deficits.

Key words: nerve regeneration, depression, functional MRI, graph theory, complex networks, brain network, classification, feature selection, NSFC grant, neural regeneration

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