中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (17): 1585-1591.doi: 10.4103/1673-5374.141783

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

迷走神经刺激创伤性脑损伤的神经保护效应

  

  • 收稿日期:2014-07-27 出版日期:2014-09-16 发布日期:2014-09-16

Neuroprotective effects of vagus nerve stimulation on traumatic brain injury

Long Zhou 1, Jinhuang Lin 1, Junming Lin 1, Guoju Kui 1, Jianhua Zhang 2, Yigang Yu 1   

  1. 1 Affiliated Dongnan Hospital of Xiamen University, the 175 Hospital of Chinese PLA, Trauma Neurosurgery Center of Nanjing Military Region, Xiamen, Fujian Province, China
    2 The 73131 Corps of Chinese PLA, Xiamen, Fujian Province, China
  • Received:2014-07-27 Online:2014-09-16 Published:2014-09-16
  • Contact: Yigang Yu, M.D., Affiliated Dongnan Hospital of Xiamen University, the 175 Hospital of Chinese PLA, Trauma Neurosurgery Center of Nanjing Military Region, Xiamen 363000, Fujian Province, China, yu-yg@tom.com.

摘要:

课题组以往研究表明刺激迷走神经可改善创伤性脑损伤的预后。本次实验旨在进一步结实迷走神经刺激对兔脑爆炸伤功能预后保护作用的机制。以连续型电刺激脑爆炸伤模型兔右侧颈迷走神经,参数为10 V,5 Hz,5 ms,20 min,检测兔血清和脑组织肿瘤坏死因子α、白细胞介素1β和白细胞介素10的表达,测定脑组织含水量。结果发现,迷走神经刺激可减轻兔脑爆炸伤后脑组织水肿程度,下调血清及脑组织内促炎因子肿瘤坏死因子α及白细胞介素1β的表达,上调抗炎因子白细胞介素10的表达。结果反映了迷走神经刺激对脑爆炸伤兔有神经保护作用,其机制为通过调节血清及脑组织内肿瘤坏死因子α及白细胞介素1β和白细胞介素10的表达来实现。

关键词: 神经再生, 脑损伤, 迷走神经刺激, 肿瘤坏死因子α, 白细胞介素1β, 白细胞介素-10, 脑组织病理, 保护, 爆炸伤, 机制, 脑水肿

Abstract:

Previous studies have shown that vagus nerve stimulation can improve the prognosis of traumatic brain injury. The aim of this study was to elucidate the mechanism of the neuroprotective effects of vagus nerve stimulation in rabbits with brain explosive injury. Rabbits with brain explosive injury received continuous stimulation (10 V, 5 Hz, 5 ms, 20 minutes) of the right cervical vagus nerve. Tumor necrosis factor-α, interleukin-1β and interleukin-10 concentrations were detected in serum and brain tissues, and water content in brain tissues was measured. Results showed that vagus nerve stimulation could reduce the degree of brain edema, decrease tumor necrosis factor-α and interleukin-1β concentrations, and increase interleukin-10 concentration after brain explosive injury in rabbits. These data suggest that vagus nerve stimulation may exert neuroprotective effects against explosive injury via regulating the expression of tumor necrosis factor-α, interleukin-1β and interleukin-10 in the serum and brain tissue.

Key words: nerve regeneration, brain injury, vagus nerve stimulation, tumor necrosis factor-α, interleukin-1β, interleukin-10, brain tissue pathology, protection, explosive injury, mechanisms, hydrocephalus, neural regeneration