中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (17): 1606-1609.doi: 10.4103/1673-5374.141786

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

牙齿缺失成年小鼠海马齿状回神经元的新生收到抑制

  

  • 收稿日期:2014-07-30 出版日期:2014-09-16 发布日期:2014-09-16
  • 基金资助:

    教育部科学技术研究重点项目(106152),兰州大学第二医院科研课题(NO.C1708)

Tooth loss inhibits neurogenesis in the dentate gyrus of adult mice

Shaochen Su 1, Tao Qi 2, Baoli Su 3, Huibin Gu 3, Jianlin Wang 1, Lan Yang 1   

  1. 1 School of Life Sciences, Lanzhou University; Second Hospital, Lanzhou University, Lanzhou, Gansu Province, China
    2 First Hospital, Lanzhou University, Lanzhou, Gansu Province, China
    3 Changzhou Institute of Mechatronic and Technology, Changzhou, Jiangsu Province, China
  • Received:2014-07-30 Online:2014-09-16 Published:2014-09-16
  • Contact: Jianlin Wang, Ph.D., School of Life Sciences, Lanzhou University; Second Hospital, Lanzhou University, Lanzhou 730030, Gansu Province, China, jlwang@lzu.edu.cn. Lan Yang, M.D., School of Life Sciences, Lanzhou University; Second Hospital, Lanzhou University, Lanzhou 730030, Gansu Province, China, ylan2005@163.com.
  • Supported by:

     This study was supported by the Science and Technology Key Project of Ministry of Education of China, No. 106152; the Scientific Research Project of Second Hospital of Lanzhou University of China, No. C1708.

摘要:

研究表明,大量牙齿缺失对小鼠学习记忆能力存在影响,可增加阿尔茨海默病的发病危险。由于海马齿状回神经元与认知功能关系密切,实验设想牙齿缺失对海马齿状回神经元有一定影响,故将成年雄性小鼠随机分为牙齿缺失组和正常对照组。牙齿缺失组拔除左侧上下颌臼齿,正常对照组不予任何干预。免疫荧光染色结果显示,造模后4周,与正常对照组相比,牙齿缺失组小鼠海马齿状回新生神经元的标志物双皮质素和成熟神经元的标志物神经元核抗原蛋白阳性表达细胞密度及蛋白免疫荧光吸收值均降低。说明牙齿缺失成年小鼠海马齿状回神经元新生受到了抑制。

关键词: 神经再生, 神经新生, 神经发生, 神经元, 牙齿缺失, 海马, 齿状回, 双皮质素, 神经元核抗原蛋白

Abstract:

Tooth loss has been shown to affect learning and memory in mice and increases the risk of Alzheimer’s disease. The dentate gyrus is strongly associated with cognitive function. This study hypothesized that tooth loss affects neurons in the dentate gyrus. Adult male mice were randomly assigned to either the tooth loss group or normal control group. In the tooth loss group, the left maxillary and mandibular molars were extracted. Normal control mice did not receive any intervention. Immunofluorescence staining revealed that the density and absorbance of doublecortin- and neuronal nuclear antigen-positive cells were lower in the tooth loss group than in the normal control group. These data suggest that tooth loss may inhibit neurogenesis in the dentate gyrus of adult mice.

Key words: nerve regeneration, neurogenesis, neurons, tooth loss, hippocampus, dentate gyrus, doublecortin, neuronal nuclear antigen, neural regeneration