中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (23): 2074-2080.doi: 10.4103/1673-5374.147934

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

传统中药葛根素保护脑缺血再灌注损伤的作用途径：抑制炎症反应

  

  • 收稿日期:2014-08-18 出版日期:2014-12-10 发布日期:2014-12-10
  • 基金资助:

    浙江省中国传统医学科学基金资助（2010za072），浙江省卫生厅基金（2012zda023）

Puerarin protects brain tissue against cerebral ischemia/reperfusion injury by inhibiting the inflammatory response

Feng Zhou 1, Liang Wang 1, 2, Panpan Liu 1, Weiwei Hu 1, Xiangdong Zhu 1, Hong Shen 1, Yuanyuan Yao 3   

  1. 1 Department of Neurosurgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, China
    2 Medical College, Ningbo University, Ningbo, Zhejiang Province, China
    3 Department of Anesthesiology, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, China
  • Received:2014-08-18 Online:2014-12-10 Published:2014-12-10
  • Contact: Yuanyuan Yao, M.D., Department of Anesthesiology, Second Affiliated Hospital,School of Medicine, Zhejiang University,Hangzhou 310009, Zhejiang Province,China, yuanyuan58@126.com.
  • Supported by:

    This study was supported by the Chinese Traditional Medical Science Foundation of Zhejiang Province in China, No. 2010ZA072; the Health Bureau Foundation of Zhejiang Province in China, No. 2012ZDA023; and the Qianjiang Project of Zhejiang Science and Technology Bureau in China, No. 2010 R10073.

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摘要：

有报道显示中药单体葛根素可在脑缺血再灌注损伤中发挥神经保护作用，实验希望揭示其作用机制，采用线栓法制作大脑中动脉缺血再灌注大鼠模型，于再灌注前30 min和再灌注后8 h腹腔内注射葛根素(100 mg/kg)，再灌注24 h后发现，葛根素能显著改善脑缺血大鼠神经缺损评分，减少脑梗死区面积和脑水含量，显著减少缺血区脑组织炎性因子Toll样受体4、髓样分化因子88、核转录因子κB和肿瘤坏死因子α的表达。说明葛根素对缺血再灌注损伤脑组织有保护作用，其作用途径下调多种炎性因子的表达发挥抗炎效应而实现其目的。

关键词: 神经再生, 脑损伤, 葛根素, 脑缺血, 再灌注损伤, 大鼠, 炎症反应, Toll样受体4, 核因子&kappa, B, 髓样分化因子88, 肿瘤坏死因子&alpha, 大脑中动脉阻塞模型

Abstract:

Puerarin, a traditional Chinese medicine, exerts a powerful neuroprotective effect in cerebral ischemia/reperfusion injury, but its mechanism is unknown. Here, we established rat models of middle cerebral artery ischemia/reperfusion injury using the suture method. Puerarin (100 mg/kg) was administered intraperitoneally 30 minutes before middle cerebral artery occlusion and 8 hours after reperfusion. Twenty-four hours after reperfusion, we found that puerarin significantly improved neurological deficit, reduced infarct size and brain water content, and notably diminished the expression of Toll-like receptor-4, myeloid differentiation factor 88, nuclear factor kappa B and tumor necrosis factor-α in the ischemic region. These data indicate that puerarin exerts an anti-inflammatory protective effect on brain tissue with ischemia/reperfusion damage by downregulating the expression of multiple inflammatory factors.

Key words: nerve regeneration, brain injury, puerarin, cerebral ischemia, reperfusion injury, rats, inflammatory reaction, Toll-like receptor-4, nuclear factor kappa B, myeloid differentiation factor 88, tumor necrosis factor-α, middle cerebral artery occlusion, neural regeneration