中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (1): 146-152.doi: 10.4103/1673-5374.150724

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

大豆甙元对大鼠局灶性脑缺血损伤的神经保护

  

  • 收稿日期:2014-11-16 出版日期:2015-01-15 发布日期:2015-01-15

Neuroprotective effects of daidzein on focal cerebral ischemia injury in rats

Adem Bozkurt Aras 1, *, Mustafa Guven 1, Tarık Akman 1, Adile Ozkan 2, Halil Murat Sen 2, Ugur Duz 3, Yıldıray Kalkan 4, Coskun Silan 5, Murat Cosar 1   

  1. 1 Department of Neurosurgery, Canakkale Onsekiz Mart University, Canakkale, Turkey
    2 Department of Neurology, Canakkale Onsekiz Mart University, Canakkale, Turkey
    3 Department of Medical Biochemistry, Ministry of Health, Administration of Public Health, Hakkari, Turkey
    4 Deparment of Histology and Embryology, Recep Tayyip Erdo?an University, Faculty of Medicine, Rize, Turkey
    5 Department of Pharmacology, Canakkale Onsekiz Mart University, Canakkale, Turkey
  • Received:2014-11-16 Online:2015-01-15 Published:2015-01-15
  • Contact: Adem Bozkurt Aras, guven2340@hotmail.com.

摘要:

鉴于植物提取物大豆甙元有抗氧化作用,实验假设大豆甙元对脑缺血有神经保护作用。给予大脑中动脉阻塞模型大鼠腹腔注射大豆甙元,生物化学和免疫组化检测结果显示,大豆甙元使模型大鼠缺血脑组织中降低的超氧化物歧化酶和核呼吸因子1水平明显升高,升高的丙二醛和凋亡相关半胱氨酸肽酶caspase-3和caspase-9水平明显下降。HE染色和牢固蓝染色检测结果显示,腹腔注射大豆甙元使缺血脑组织中神经元损害明显减轻。说明大豆甙元干预可通过降低氧自由基水平,对缺血脑组织有神经保护作用,验证了前述假设。

关键词: 神经再生, 脑缺血, 脑卒中, 大豆甙元, 氧化应激, 凋亡, 超氧化物歧化酶, 丙二醛, 核呼吸因子

Abstract:

Daidzein, a plant extract, has antioxidant activity. It is hypothesized, in this study, that daidzein exhibits neuroprotective effects on cerebral ischemia. Rat models of middle cerebral artery occlusion were intraperitoneally administered daidzein. Biochemical and immunohistochemical tests showed that superoxide dismutase and nuclear respiratory factor 1 expression levels in the brain tissue decreased after ischemia and they increased obviously after daidzein administration; malondialdehyde level and apoptosis-related cysteine peptidase caspase-3 and caspase-9 immunoreactivity in the brain tissue increased after ischemia and they decreased obviously after daidzein administration. Hematoxylin-eosin staining and luxol fast blue staining results showed that intraperitoneal administration of daidzein markedly alleviated neuronal damage in the ischemic brain tissue. These findings suggest that daidzein exhibits neuroprotective effects on ischemic brain tissue by decreasing oxygen free radical production, which validates the aforementioned hypothesis.

Key words: nerve regeneration, brain ischemia, stroke, daidzein, oxidative stress, apoptosis, superoxide dismutase, malondialdehyde, nuclear respiratory factor 1, neural regeneration