中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (3): 412-418.doi: 10.4103/1673-5374.153689

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

3.0T核磁共振弥散张量成像可用于评估大鼠完全性脊髓损伤功能的恢复

  

  • 收稿日期:2014-08-18 出版日期:2015-03-20 发布日期:2015-03-20
  • 基金资助:

    陕西省科学技术研究发展项目（2013K12-20-08）

Feasibility of 3.0 T diffusion-weighted nuclear magnetic resonance imaging in the evaluation of functional recovery of rats with complete spinal cord injury

Duo Zhang 1, Xiao-hui Li 2, Xu Zhai 1, Xi-jing He 1   

  1. 1 Second Department of Orthopedics, Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi Province, China
    2 Department of Radiology, Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi Province, China
  • Received:2014-08-18 Online:2015-03-20 Published:2015-03-20
  • Contact: Xi-jing He, M.D., Ph.D., xijing_h@vip.tom.com
  • Supported by:

    This study was financially supported by a grant from the Shaanxi Provincial Science and Technology Research and Development Project, No. 2013K12-20-08

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摘要：

弥散张量成像能够敏感地反映脊髓损伤后轴突坏死、变性、胶质细胞再生、脱髓鞘等变化，显示活体脊髓的微观结构变化。发展的弥散张量成像技术对脊髓损伤的诊断更加敏感，后期的纤维示踪成像图能够将白质纤维可视化，直观显示纤维束的完整性及受损情况。但目前弥散张量成像仅在脑部检查中广泛应用，较少应用于脊髓损伤的评价。实验结合评价脊髓功能恢复常用平均联合得分，希望明确探讨核磁共振弥散张量成像的各向异性分数和表观扩散系数两项参数及其纤维束示踪对大鼠完全性脊髓损伤评价的可行性。结果显示，大鼠脊髓横断后平均联合得分明显降低，而后随时间的延长而逐渐增加。大鼠脊髓横断后各向异性分数值下降，脊髓横断后均明显低于正常大鼠（P<0.05）；表观扩散系数值增高，明显高于正常组（P<0.05）；脊髓横断后各向异性分数值与表观扩散系数值呈负相关（r=-0.856，P<0.01）。脊髓横断后各向异性分数值与平均联合得分呈正相关（r=0.943，P<0.01），表观扩散系数值与平均联合得分呈负相关（r=-0.949，P<0.01）。实验结果提示，作为无创性检查，核磁共振弥散张量成像能够为脊髓损伤提供定性和定量的信息，其各向异性分数值和表观扩散系数值与平均联合得分评分有较好的相关性，能够反映脊髓功能的恢复情况。

关键词: 神经再生, 脊髓损伤, 脊髓横断, 平均联合得分, MRI, 弥散张量成像, 各向异性分数, 表观扩散系数, 纤维束示踪

Abstract:

Diffusion tensor imaging is a sensitive way to reflect axonal necrosis and degeneration, glial cell regeneration and demyelination following spinal cord injury, and to display microstructure changes in the spinal cord in vivo. Diffusion tensor imaging technology is a sensitive method to diagnose spinal cord injury; fiber tractography visualizes the white matter fibers, and directly displays the structural integrity and resultant damage of the fiber bundle. At present, diffusion tensor imaging is restricted to brain examinations, and is rarely applied in the evaluation of spinal cord injury. This study aimed to explore the fractional anisotropy and apparent diffusion coefficient of diffusion tensor magnetic resonance imaging and the feasibility of diffusion tensor tractography in the evaluation of complete spinal cord injury in rats. The results showed that the average combined scores were obviously decreased after spinal cord transection in rats, and then began to increase over time. The fractional anisotropy scores after spinal cord transection in rats were significantly lower than those in normal rats (P < 0.05); the apparent diffusion coefficient was significantly increased compared with the normal group (P < 0.05). Following spinal cord transection, fractional anisotropy scores were negatively correlated with apparent diffusion coefficient values (r = –0.856, P < 0.01), and positively correlated with the average combined scores (r = 0.943, P < 0.01), while apparent diffusion coefficient values had a negative correlation with the average combined scores (r = –0.949, P < 0.01). Experimental findings suggest that, as a non-invasive examination, diffusion tensor magnetic resonance imaging can provide qualitative and quantitative information about spinal cord injury. The fractional anisotropy score and apparent diffusion coefficient have a good correlation with the average combined scores, which reflect functional recovery after spinal cord injury.

Key words: nerve regeneration, spinal cord injury, spinal cord transection, average combined score, magnetic resonance imaging, diffusion tensor imaging, fractional anisotropy, apparent diffusion coefficient, fiber tractography, neural regeneration