中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (4): 605-609.doi: 10.4103/1673-5374.155435

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

葛根素在体外培养神经元中分布和保留时间比在星形胶质细胞中更多更长

  

  • 收稿日期:2015-01-22 出版日期:2015-04-22 发布日期:2015-04-22
  • 基金资助:

    国家自然科学基金(Grant No. 31402237, 81473549)，中央高校基本科研基金(Grant No. XDJK2014C058, XDJK2014D023, XDJK2015D016)；国家重点新药开发项目(Grant No. 2014ZX09304-306-04)，重庆基础与前沿研究基金(Grant No. CSTC2014jcyjA80023)，重庆自然科学基金(Grant No. CSTC2012jjA10012)

Puerarin exhibits greater distribution and longer retention time in neurons than astrocytes in a co-cultured system

Shu-Yong Wei 1, 2, Jie Tong 1, Qiang Xue 1, Fang-hong Shang 1, Yan-jun Li 1, Yang Liu 1, Bin-bin Feng 3, Xiao-yu Xu 1   

  1. 1 Pharmaceutical Sciences College & Chinese Medicine College of Southwest University; Chongqing Engineering Research Center for
    Pharmacodynamics Evaluation, Chongqing, China
    2 Rongchang Campus of Southwest University, Chongqing, China
    3 Chongqing Three Gorges Medical College, Chongqing, China
  • Received:2015-01-22 Online:2015-04-22 Published:2015-04-22
  • Contact: Xiao-yu Xu, xuxiaoyu@swu.edu.cn.
  • Supported by:

    This study was supported by the National Natural Science Foundation of China, No. 31402237, 81473549; the Fundamental Research Funds for Central Universities in China, No. XDJK2014C058, XDJK2014D023, XDJK2015D016; a grant from the National Key New Drug Development Project of China, No. 2014ZX09304-306-04; the Fundamental and Front Research Funds of Chongqing of China, No. CSTC2014jcyjA80023; and a grant from the Natural Science Foundation of Chongqing of China, No. CSTC2012jjA10012.

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摘要：

葛根素对阿尔茨海默病、帕金森病和脑缺血的神经保护作用已在体内实验中得到证实，但其对体外培养的神经元和星形胶质细胞是否具有相同的神经保护作用尚少见报道。实验将新生SD大鼠大脑皮质的神经元和星形胶质细胞共培养于基于Transwell膜的共培养系统中。应用荧光分光光度法和荧光显微镜检测葛根素在这两种细胞共培养及单独培养过程中的保留时间及分布。葛根素在2种细胞共培养及单独培养时，均表现为在神经元中的浓度高于星形胶质细胞。在加入葛根素20min后，药物浓度达到峰值；而在葛根素加入60min后，星形胶质细胞中几乎检测不到该药。葛根素在单独和共培养的神经元中的浓度达到稳定的12.8 ng/mL。结果表明葛根素在体外培养的神经元中分布和保留时间均优于星形胶质细胞。

关键词: 神经再生, 葛根素, 体外实验, 共培养, 神经元, 星形胶质细胞, Transwell小室, 新生大鼠

Abstract:

The phytoestrogen puerarin has been shown to protect neurons and astrocytes in the brain, and is therefore an attractive drug in the treatment of Alzheimer’s disease, Parkinson’s disease and cerebral ischemia. Whether puerarin exhibits the same biological processes in neurons and astrocytes in vitro has rarely been reported. In this study, cortical neurons and astrocytes of newborn Sprague-Dawley rats were separated, identified and co-cultured in a system based on Transwell membranes. The retention time and distribution of puerarin in each cell type was detected by fluorescence spectrophotometry and fluorescence microscope. The concentration of puerarin in both co-cultured and separately cultured neurons was greater than that of astrocytes. Puerarin concentration reached a maximum 20 minutes after it was added. At 60 minutes after its addition, a scant amount of drug was detected in astrocytes; however in both separately cultured and co-cultured neurons, the concentration of puerarin achieved a stable level of about 12.8 ng/mL. The results indicate that puerarin had a higher concentration and longer retention time in neurons than that observed in astrocytes.

Key words: nerve regeneration, puerarin, in vitro experiments, co-culture, neurons, astrocytes, Transwell, neonatal rats, neural regeneration