中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (4): 636-643.doi: 10.4103/1673-5374.155440

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

丙泊酚联合骨髓间充质干细胞移植脊髓损伤大鼠：改善后肢电生理功能作用优于单独用药

  

  • 收稿日期:2015-03-06 出版日期:2015-04-22 发布日期:2015-04-22

Propofol injection combined with bone marrow mesenchymal stem cell transplantation better improves electrophysiological function in the hindlimb of rats with spinal cord injury  than monotherapy

Yue-xin Wang 1, Jing-jing Sun 2, Mei Zhang 3, Xiao-hua Hou 1, Jun Hong 4, Ya-jing Zhou 5, Zhi-yong Zhang 1   

  1. 1 First Department of Orthopedics, Tangshan Worker’s Hospital Affiliated to Hebei Medical University, Tangshan, Hebei Province, China
    2 Department of Neurology, Tangshan Union Medical College Hospital, Tangshan, Hebei Province, China
    3 Operating Room, Tangshan Worker’s Hospital Affiliated to Hebei Medical University, Tangshan, Hebei Province, China
    4 Department of Neurosurgery, Tangshan Worker’s Hospital Affiliated to Hebei Medical University, Tangshan, Hebei Province, China
    5 Department of Anesthesiology, Xingtai People’s Hospital Affiliated to Hebei Medical University, Xingtai, Hebei Province, China
  • Received:2015-03-06 Online:2015-04-22 Published:2015-04-22
  • Contact: Zhi-yong Zhang, zhangzhiyongho@sina.com

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摘要：

骨髓间充质干细胞移植对神经系统损伤的修复作用仍不理想；而有研究发现丙泊酚对脊髓损伤起保护作用。因此实验拟探索两者联合对脊髓损伤的治疗效果。以打击法建立脊髓损伤大鼠模型后，分别以骨髓间充质干细胞尾静脉注射移植和/或丙泊酚尾静脉泵注方法进行治疗。发现经骨髓间充质干细胞移植和/或丙泊酚治疗后4周，脊髓损伤大鼠脊髓空洞明显缩小，脊髓组织中PKH-26阳性细胞和辣根过氧化物酶阳性神经纤维数量明显增加，下肢感觉诱发电位及运动诱发电位的潜伏期明显缩短，波幅增加，下肢运动功能明显改善，且两者联合效果优于单独使用。表明丙泊酚联合骨髓间充质干细胞移植治疗可有效改善大鼠脊髓损伤后肢电生理功能，促进其运动功能的恢复，起到神经保护作用。

关键词: 神经再生, 骨髓间充质干细胞, 丙泊酚, 脊髓损伤, 细胞移植, 电生理, 后肢功能, 干细胞, 神经保护

Abstract:

The repair effects of bone marrow mesenchymal stem cell transplantation on nervous system damage are not satisfactory. Propofol has been shown to protect against spinal cord injury. Therefore, this study sought to explore the therapeutic effects of their combination on spinal cord injury. Rat models of spinal cord injury were established using the weight drop method. Rats were subjected to bone marrow mesenchymal stem cell transplantation via tail vein injection and/or propofol injection via tail vein using an infusion pump. Four weeks after cell transplantation and/or propofol treatment, the cavity within the spinal cord was reduced. The numbers of PKH-26-positive cells and horseradish peroxidase-positive nerve fibers apparently increased in the spinal cord. Latencies of somatosensory evoked potentials and motor evoked potentials in the hindlimb were noticeably shortened, amplitude was increased and hindlimb motor function was obviously improved. Moreover, the combined effects were better than cell transplantation or propofol injection alone. The above data suggest that the combination of propofol injection and bone marrow mesenchymal stem cell transplantation can effectively improve hindlimb electrophysiological function, promote the recovery of motor funtion, and play a neuroprotective role in spinal cord injury in rats.

Key words: nerve regeneration, bone marrow mesenchymal stem cells, propofol, spinal cord injury, cell transplantation, electrophysiology, motor function, stem cells, neuroprotection, neural regeneration