中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (6): 958-964.doi: 10.4103/1673-5374.158361

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

急性脊髓损伤2周内蛛网膜下腔注射富氢盐水可促进修复?

  

  • 收稿日期:2015-05-07 出版日期:2015-06-18 发布日期:2015-06-18

Hydrogen-rich saline injection into the subarachnoid cavity within 2 weeks promotes recovery after acute spinal cord injury

Jian-long Wang, Qing-shan Zhang, Kai-di Zhu, Jian-feng Sun, Ze-peng Zhang, Jian-wen Sun, Ke-xiang Zhang   

  1. Department of Orthopedics, the Third Xiangya Hospital of Central South University, Changsha, Hunan Province, China
  • Received:2015-05-07 Online:2015-06-18 Published:2015-06-18
  • Contact: Ke-xiang Zhang, Ph.D. candidate,xy3hzkx@126.com.
  • Supported by:

    This study was supported by a grant from Hunan Provincial Science and Technology Ministry of China, No. 2015JJ6116.

摘要:

氢可有效对抗介导组织损伤的氧化应激、炎症反应及细胞凋亡过程,已有研究证实,注射含治疗剂量氢的富氢盐水是一种有效的输送分子氢的方法。实验假设富氢盐水对Allen打击脊髓损伤模型大鼠有促进修复的作用。于脊髓损伤后0.5,1,2,4,8,12,24 h,预先置导管于大鼠蛛网膜下腔,用此导管将富氢盐水以0.25 mL/kg输注,每天以相同方式注药1次至2周。结果显示,大鼠脊髓损伤后24,48 h,1,2周,富氢盐水均显著减轻了损伤脊髓细胞死亡及炎细胞浸润,降低了血清丙二醛水平以及损伤脊髓组织半胱氨酸蛋白酶3的免疫反应,提高了血清超氧化物歧化酶活性及损伤脊髓组织降钙素基因相关肽免疫反应,并改善了模型大鼠后肢运动功能。结果证实,在脊髓损伤后2周内采用富氢盐水干预,可有效促进损伤脊髓急性期的修复。

关键词: 神经再生, 脊髓损伤, 富氢盐水, 生理盐水, 活性氧, 氧化应激, BBB评分, 丙二醛, 超氧化物歧化酶, 降钙素基因相关肽, caspase-3

Abstract:

Hydrogen can relieve tissue-damaging oxidative stress, inflammation and apoptosis. Injection of hydrogen-rich saline is an effective method for transporting molecular hydrogen. We hypothesized that hydrogen-rich saline would promote the repair of spinal cord injury induced by Allen’s method in rats. At 0.5, 1, 2, 4, 8, 12 and 24 hours after injury, then once daily for 2 weeks, 0.25 mL/kg hydrogen-rich saline was infused into the subarachnoid space through a catheter. Results at 24 hours, 48 hours, 1 week and 2 weeks after injury showed that hydrogen-rich saline markedly reduced cell death, inflammatory cell infiltration, serum malondialdehyde content, and caspase-3 immunoreactivity, elevated serum superoxide dismutase activity and calcitonin gene-related peptide immunoreactivity, and improved motor function in the hindlimb. The present study confirms that hydrogen-rich saline injected within 2 weeks of injury effectively contributes to the repair of spinal cord injury in the acute stage.

Key words: nerve regeneration, spinal cord injury, hydrogen-rich saline, reactive oxygen species, physiological saline, oxidative stress, Basso, Beattie and Bresnahan score, malondialdehyde, superoxide dismutase, calcitonin gene-related peptide, caspase-3, neural regeneration